Abstract In vitro cucumber haploid line generation in several new cultivars

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As. Pac. J. Mol. Biol. & Biotech., Jan 2013 Vol. 1, 19-28

In vitro cucumber haploid line generation in several new cultivars

E. Moqbeli, Gh. Peyvast, Y. Hamidoghli and J.A. Olfati*

Horticultural Department, Faculty of Agriculture, University of Guilan, , Rasht, Iran. I.R

* Author for correspondence: J.A. Olfati
Horticultural Department, Faculty of Agriculture, University of Guilan, Rasht, Iran. I.R.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Due to recent developments, pure lines can now be produced in a short time using in vitro techniques and therefore reduce by several years the time required for conventional plant breeding programmes. In the current study, six F1 cucumber hybrids were investigated, namely ‘Kashmir’, ‘Adergreen’, ‘Summerstar’, ‘Royal’, ‘2010-3’ and ‘502×605’. Unfertilized ovaries were harvested and placed on solid MS medium in Petri dishes. Immediately after placing the unfertilized ovary slices of each variety on induction medium containing different concentrations of Thidiazuron (TDZ) (0, 0.01, 0.02, 0.03 and 0.04 mg∙L-1), they were exposed to a thermal shock pretreatment at 35±1°C for 0, 2, 3 or 4 days. The first visual structures formed after 3 weeks in culture. Cultivar, TDZ concentration and temperature pretreatment all had a significant effect on cucumber embryogenesis. The greatest embryogenesis success was obtained in the ‘summerstar’ variety cultured in medium with 0.04 mg∙L-1 TDZ. TDZ also had a positive effect on embryo formation in our work.

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Abstract In vitro propagation of Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. via asymbiotic seed germination

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As. Pac. J. Mol. Biol. & Biotech., Jan 2013 Vol. 1, 29-34

In vitro propagation of Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. via asymbiotic seed germination

P. Servin Wesley1, B. Chitra Devi2*, B. Sahaya Shibu1 and Sarmad Moin1

1Department of Biotechnology, Karpagam University, Coimbatore, Tamilnadu, India.
2Department of Botany, Karpagam University, Coimbatore, Tamilnadu, India.

* Author for correspondence: B. Chitra Devi
Assistant Professor, Department of Botany, Karpagam University, Coimbatore, Tamilnadu, India.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

The application of plant tissue culture techniques for conservation and propagation of many threatened species requires an efficient in vitro regeneration protocol. In this study, various basal media were evaluated to determine their effectiveness in promoting asymbiotic seed germination of three important orchid species, Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. Five different basal media, Murashige and Skoog medium (MS), Linsmaier and Skoog medium (LS), Lindemann orchid medium (LOM), Schenk and Hildebrandt medium (SH) and Knudson C medium (KC) were evaluated, among which LS and LOM elicited a better response from all the three orchids. Maximum germination of Coelogyne breviscapa and Flickingeria nodosa seeds was observed on LOM and for Dendrobium aqueum LS medium elicited a better response.

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Abstract The versatility of comparative genomics in the post genomic era

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As. Pac. J. Mol. Biol. & Biotech., Dec 2001 Vol. 9(1) : 1-6

The versatility of comparative genomics in the post genomic era

Philippa Melamed and Boon Chuan Low*

Department of Biological Sciences, National University of Singapore, Blk S2, 14 Science Drive 4, Singapore 117543

(Received 22 January 2001 / Accepted 30 March 2001)

Abstract.
The biggest challenge scientists are facing in this post genomic era is to define the hidden messages in the strings of genetic codes. The best and easiest route to achieve this is to compare the genomic contents of various species and methodically sieve through the information to correlate what is "common" and what is "unique". The tool of comparative genomics has allowed us to explore various disciplines in life sciences, ranging from the basic understanding of evolution and systematics, developmental and cell biology, protein function, to the applications of development in drug discovery and pharmacogenomics as well as environmental protection. This review will highlight a few key areas where such applications are now being pursued with good promises and reinforces the need to develop comparative genomics as a multi disciplinary tool.

Keywords: biotechnology, comparative genomics, evolution, pharmacogenomics, proteins

Abstract Novel application of coconut husk as growth support matrix and natural inducer of fungal laccase production in a bubble column reactor

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As. Pac. J. Mol. Biol. & Biotech., April 2009 Vol. 17, 39-44

Novel application of coconut husk as growth support matrix and natural inducer of fungal laccase production in a bubble column reactor

Muhd Alimin Abdul Karim and Mohamad Suffian Mohamad Annuar

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

*Author for Correspondence.
Institute of Biological Sciences,
Faculty of Science, University of Malaya,
Kuala Lumpur 50603, Malaysia.
Tel: 6-03-7967-6740, Fax: 6-03-7967-4178,
E-mail: suffian_ This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract.
Laccase production by a white-rot fungus Pycnoporus sanguineus and its growth in a bubble column reactor were studied as a function of different inducers and superficial gas velocities. Free suspended biomass and immobilized biomass systems were studied where in the latter novel application of coconut husk as simultaneous support matrix and natural laccase inducer was studied. Good biomass growth was observed in the free suspension cultivation but no laccase activity was detected even after known enzyme inducers were applied. In the immobilized biomass system supplied with aeration at superficial gas velocity of 1.14 x 10-3 m s-1, the fungus growing on the surface of coconut husk produced significant amount of biomass at 2.4 ± 0.2 g L-1 dry weight as compared to the non-aerated (control) system where only 0.6 ± 0.1 g L-1 dry weight of biomass was obtained. Laccase activity was detected only after 36 hours of fermentation and the highest activity i.e. 2.85 ± 0.05 U L-1 was obtained at 48 hours. No laccase activity was detected in the non-aerated system even after 48 hours of fermentation.

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Abstract Production Of Fused Protein Consisting Of Vp2 From Infectious Bursal Disease Virus And Hn From Newcastle Disease Virus In Escherichia Coli

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Production Of Fused Protein Consisting Of Vp2 From Infectious Bursal Disease Virus And Hn From Newcastle Disease Virus In Escherichia Coli

Muhammad N.A.A.N.1, *Ahmad-Raus R.2, Amin N.M.3, Syamsiah A. S.4, Bakar F.D.A.5, Ghazali M.S.1

1Department of Biomedical Science, Faculty of Health Sciences, International Islamic University Malaysia
2Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia
3Malaysian Agricultural Research and Development Institute, Serdang, Malaysia
4Veterinary Research Institute, Ipoh, Malaysia
5School of Bioscience and Biotechnology, National University of Malaysia

* Author for correspondence: Raha Ahmad-Raus
Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University of Malaysia, Jalan Gombak, Selangor
Tel.:+603-61964588 E-mail address: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Viral protein 2 (VP2) of infectious bursal disease virus (IBDV) and hemagglutinin-neuraminidase (HN) of Newcastle disease virus (NDV) are viral surface proteins which contain epitopes that are able to induce neutralizing antibodies for protection against Newcastle disease and infectious bursal disease. In the present study, recombinant fused protein consisting of VP2 and partial HN protein was produced. The fused protein was made by fusing VP2 gene to full length (flHN) and partial HN (pHN) gene, separately and cloned it into pRSETB expression vector. The recombinant construct is then transformed into E. coli BL21 (DE3) for production of the fused protein. The fused VP2-pHN protein was successfully produced in E. coli BL21 (DE3) strain with the size of 75 kDa detected by anti-His monoclonal antibody via Western blot analysis.

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