Abstract Tissue Culture Propagation and Dispatch of Quality Teak Clones

Category: Uncategorised

As. Pac. J. Mol. Biol. & Biotech., January 2010 Vol. 18, 147-149

Tissue Culture Propagation and Dispatch of Quality Teak Clones

Doreen KS Goh1*, Felicia Chang1, Malcolm Jilimin1 and Yani Japarudin2

1Biotechnology and Horticulture Division, Yayasan Sabah Group, Voluntary Association Complex, Mile 2 ½, Off Jalan Tuaran, P. O. Box 11623, 88817 Kota Kinabalu, Sabah, Malaysia.
2Sabah Softwoods Sdn Bhd, Km 8, Jalan Sin San, Pasir Putih, Sabah, Malaysia.

*Author for Correspondence.
Biotechnology and Horticulture Division, Yayasan Sabah Group, Voluntary Association Complex, Mile 2 ½, Off Jalan Tuaran, P. O. Box 11623, 88817 Kota Kinabalu, Sabah, Malaysia. Tel: 6088-263185,
Fax: 6088-263424, E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract.
The Plant Biotechnology Laboratory, operating under the Biotechnology and Horticulture Division, Sabah Foundation Group, was set up in the early 1990s as part of the plant improvement program jointly undertaken by ICSB and CIRAD-Foret, a French R & D organization. Special efforts have been devoted to vegetative propagation strategies based on the development of efficient techniques at both the laboratory and nursery levels. The primary species of interest is on teak, the most valuable timber species in the world today and as such, very much sought after. The determining strategy was to develop efficient nursery and in vitro techniques for clonally mass-propagating true-to-type “Plus” trees, the selection of which is constantly upgraded from genetically highly diverse base populations. With the possibility to overcome the constraints of phytosanitary certification, tissue-culture issued clonal materials have been successfully dispatched to various oversea destinations. Data collected to date have shown that our selected Solomon Island teak origins are adaptable to a wide range of environmental conditions, demonstrating their superiority with respect to traits of major economical value over seed-issued plants from local origins. Our selected plant materials are also characterized by DNA markers and wood analyses for enhanced quality and property right control, these being taken into consideration in the early stage of the selection process.

[Get pdf]

Abstract Factor VIII gene deletion in a Thai hemophilia A patient

Category: Uncategorised

As. Pac. J. Mol. Biol. & Biotech., Dec 1998 Vol. 6(2) : 115-120

Factor VIII gene deletion in a Thai hemophilia A patient

P. Pung amritt', A. Treesucon', S. Petrarat', G. Veerakul, C. Mahasandana' and P.T. Yenchitsornanus'*

Hematology Unit, Department of Pediatrics, 'Division of Medical Genetics, Department of Medicine, 'Molecular Genetic and Medical Molecular Biology Units, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand

(Received 20 January 1998 / Accepted 3] July 1998)

Abstract.
Hemophilia A is a common X linked recessive bleeding disorder caused by abnormal factor VIII gene, resulting in deficiency of clotting factor VIII protein. The factor VIII gene consists of 26 exons and spans about 186 kilobase DNA. Different types of mutations in the factor VIII gene including inversions, point mutations, deletions, and insertions have been described. The most common mutation in severe hemophilia A patients is the inversion involving exons 1-22 of the factor VIII gene. Mutations causing hemophilia A in Thai patients are being characterized with the aim to understand molecular defects of the disease and to develop direct methods for carrier detection and prenatal diagnosis by DNA technique. Here, we report the first example of the factor VIII gene deletion found in a severe Thai hemophilia A patient. The gene deletion in this patient was observed from screening DNA samples of Thai hemophilia A patients with multiplex PCR developed to simultaneously amplify DNA sequences in 7 exons of the gene. The extent of factor VIII gene deletion in this patient was found to involve DNA region of about 2.2 kb covering exon 26 and the 3' non coding sequence of the factor VIII gene.

Keywords: factor VIII, gene deletion, hemophilia, multiplex PCR, mutation, Thai

Abstract Application of cold pretreatment and optimisation of media for enhancement of anther culture response in two barley (Hordeum vulgare L.) genotypes derived from Bangladesh

Category: Uncategorised

As. Pac. J. Mol. Biol. & Biotech., Jan 2014 Vol. 1, 127-136

Application of cold pretreatment and optimisation of media for enhancement of anther culture response in two barley (Hordeum vulgare L.) genotypes derived from Bangladesh

Mozidul Haque and S. M. Shahinul Islam*

Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.

* Author for correspondence: Dr. S. M. Shahinul Islam
Associate Professor, Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Rapid development of embryoids and the efficiency of regeneration by anther culture was observed in two barley genotypes. Five semi-solid media viz. MS, B5, N6, FHG and AMS3 were used for embryo induction as well as plant regeneration. Harvested spikes were pretreated with cold for 2, 4, 6, 8, 10, 12, 14 or 16 days at 4°C in the dark. Cold pretreatment for 8-12 days produced the highest frequency of embryoid induction on FHG medium. The FHG medium was modified by MS that was supplemented with 2,4-D (2.0 mg/l), kinetin (0.5 mg/l) and an amino acid (730 mg/l L-glutamine). Out of two genotypes, BB-6 performed better in terms of embryo formation and green plant regeneration in MSR medium. The highest level of embryoid induction (14.6%) was observed at T5 (10 days), producing a total of 13.8% regenerated plantlets and 10.72% green plants in BARI barley-6. Analysis of variance (ANOVA) showed significant differences in embryoids induction rate depending on the duration of cold pretreatment, culture media and between the genotypes. It was observed that the interactions of medium and cold pretreatment, and genotype and medium, were significant in determining the rate of embryoid induction. Cold pretreatment for 10 days of excised anthers from spikes produced optimum results in both genotypes, and the FHG medium was the best out of the five tested for embryoid induction and plant regeneration.

[Get pdf]

Abstract Effect of silver nitrate and gibberellic acid on in vitro regeneration, flower induction and fruit development in Naga Chilli

Category: Uncategorised

As. Pac. J. Mol. Biol. & Biotech., Jan 2014 Vol. 1, 137-144

Effect of silver nitrate and gibberellic acid on in vitro regeneration, flower induction and fruit development in Naga Chilli

G. Bora1,2*, H. K. Gogoi1, P. J. Handique2

1Division of Biotechnology, Defence Research Laboratory, Tezpur-784001, Assam, India.
2Department of Biotechnology, Gauhati University, Guwahati-781014, India.

* Author for correspondence: Ms. Geetashree Bora
Christian Colony, Jail Road, Borbheta, Jorhat-785004, Assam, India.
Tel : 91-7896851882 Email : This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

In vitro flower induction and fruit development are rarely achieved in the genus Capsicum. This communication reports for the first time the induction of in vitro flower induction and fruit development in Bhot jolokia or Naga Chilli under the influence of silver nitrate (AgNO3) and gibberellic acid (GA3). The greatest number of multiple shoots from a single shoot apical meristem was induced in MS medium fortified with BAP (45µML-1), NAA (5.5µML-1) and AgNO3 (35µML-1). Half strength MS medium supplemented with AgNO3 at a concentration of 34µML-1 and GA3 at 28µML-1 was found to be the optimum concentration for in vitro flower induction, and AgNO3 at 32µML-1 added to half strength MS was found best for in vitro fruit development. In vitro regenerated shoots were provided with rooting medium containing MS medium, IBA and BAP at 3.5μML-1 and 2.5μML-1, respectively. Healthy rooted plantlets were transferred to potting substrate where half strength MS medium was enhanced with soil and vermicompost in equal volumes (1:1) for hardening in the poly house. 72.6% of all acclimatised plants were successfully transferred to the open field.

[Get pdf]

Abstract Functional isocitrate Iyase gene promoter of cucumber on GUS expression in germinating seeds of transgenic tobacco

Category: Uncategorised

As. Pac. J. Mol. Biol. & Biotech., Dec 2000 Vol. 8(2) : 107-114

Functional isocitrate Iyase gene promoter of cucumber on GUS expression in germinating seeds of transgenic tobacco

1. Ismail*1, L.D. Bellis1,2 and S.M. Smith1

1Institute of Cell and Molecular Biology, University of Edinburgh, the King's Buildings,
Mayfield Road, Edinburgh, EH9 3JH, United Kindom;
2Dipartimento di Biologia Piante Agrarie, sez. Fisiologia Vegetale, Universita di Pisa,
via Mariscoglia 34, 56124 Pisa Italy

Received 16 June 2000 / Accepted 14 September 2000

Abstract.
A series of cucumber isocitrate lyase (ICL) gene promoter linked to GUS were introduced into Nicotiana plumbaginifolia by leaf disc transformation using Agrobacterium tumefaciens. The plants were self fertilised and seeds germinated for GUS assay. With 2900 bp ICL promoter, the GUS activity rose from stage 0 to its peak at stage 4 and then fell at stage 7. Deletion to 1568 bp greatly reduced the GUS activity suggesting for a cis acting sequences between the two regions required for seeds germination. Further deletions of ICL promoter retained a low level of GUS activity. Nevertheless, GUS histochemical assay showed that constructs with ICL promoter of 1568 lip, 1367 bp, 1193 and 572 bp directed GUS expression suggesting a qualitative germination response. All of the ICL promoter constructs caused organ specific pattern of GUS expression in cotyledons and expression was also observed in the root hairs of the seedlings.

Keywords: Agrobactetium tumefaciens, isocitrate lyase promoter, GUS, germinating seeds, tobacco

More Articles ...

Sponsors Members

  • image
  • image
  • image
  • 1

About MSMBB

We are a non-profit organisation that was established in 1988 to promote molecular biology and biotechnology.

Stay Connected on:

Contact Us

For general information about MSMBB, including registration, please contact us at:

  Department of Parasitology,
Faculty of Medicine,
University of Malaya,
50603 Kuala Lumpur,
Malaysia.
  This email address is being protected from spambots. You need JavaScript enabled to view it.
  +603 - 7967 4744
  +603 - 7967 4749