As. Pac. J. Mol. Biol. & Biotech., January 2010 Vol. 18, 225-228
| Agrobacterium-mediated transformation of Vanda using protocorm-like bodies Bipna Rani Shrestha1, Dong Poh Chin1, Ken Tokuhara2, Masahiro Mii1* 1Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan 2Research and Development Center, Orchid Santuary Dogashima, Nishina, Nishiizu-cho, Kamo-gun, Shizuoka 410-3514, Japan *Author for Correspondence. Abstract. |
As. Pac. J. Mol. Biol. & Biotech., Dec 1998 Vol. 6(1) : 47-54
| Observation of nuclear division in Naegleria gruberi under fluorescence microscopy Nakisah Mat Amin* and 1Keith Vickerman Unit of Biological Sciences, Faculty of Science and Professional Arts, Universiti Putra Malaysia Terengganu, Mengabang Telipot 21030 Kuala Terengganu, Malaysia, 1Division of Environmental Evolutionary Biology, Graham Kerr Building, University of Glasgow, G12 8QQ, Scotland, UK. (Received 9 January 1998 / Accepted 23 February 1998) Abstract. Keywords: Anti a tubulin, 4', 6 diamidino 2 phenylindole DAP1) Propidium Iodide (PI), Centrosome, Spindle Microtubules |
As. Pac. J. Mol. Biol. & Biotech., January 2010 Vol. 18, 111-114
| Effects of Some Cytokinins, Auxins and Medium Constituents on In Vitro Propagation of Polystachya sp. Chamchuree Sotthikul1,3* Parichat Choomporn2, Siriwan Kammuen1 and Chuntana Suwanthada1,3 1Department of Plant Science and Natural Resources, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. 2H.M.the King’s Initiative Centre for Flower and Fruit Propagation, c/o Chiang Mai University, Thailand. 3Huai Hong Khrai Royal Development Study Centre, Doi Saket, Chiang Mai 52200, Thailand. *Author for Correspondence. Abstract. |
As. Pac. J. Mol. Biol. & Biotech., Dec 1999 Vol. 7(1) : 53-60
| Isolation and characterisation of a cDNA encoding I aminocyclopropane l carboxylate oxidase from mango (Mangifera indica L.) Z. Zainal*, G. A. Tucker and G. W. Lycett Department ofPhysiology and Environmental Science, Faculty of Agricultural and Food Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire. LE12 5RD, UK (Received 15 February 1999 / Accepted 28 May 1999) Abstract. Keywords: Gene expression, Mangifiera indica, ACC oxidase, cDNA, fruit ripening |
As. Pac. J. Mol. Biol. & Biotech., Apr 2014 Vol. 2, 185-190
A simple in vitro method to study alkaline tolerance in alkaligrass (Puccinellia airoides) at high pH
Xue Zhang1, Koji Nomura*1, Katsuyoshi Shimizu2 and Ke-Zhang Xu3
1Graduate School of Life and Environmental Science, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan,
2Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan,
3Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, Jilin Province, China.
* Author for correspondence: Koji Nomura
Graduate School of Life and Environmental Science, University of Tsukuba Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it. Tel.: +81-29-853-6692 Fax: +81-29-853-6692
Abstract.
Species belonging to genus Puccinellia are commonly called 'alkaligrass' because of their tolerance to saline-alkali environment. Although phyto-remediation of saline-alkali lands by planting alkaligrass is already achieved, its mechanisms of alkali tolerance need to be further comprehensively and thoroughly investigated. In a previous work, we revealed that alkaligrass had high tolerance to a high pH condition already during its germination and initial growth by growing seedlings in vitro. For further studies of alkali tolerance, an in vitro-culture system under a sterile condition is required. In a previous work, we cultured young alkaligrass seedlings on an agar medium to reveal their responses to a high pH stress. In the present work, we elaborated the in vitro method further and developed a convenient, efficient and reliable culture system. We compared the roots of the seedlings that were grown in the in vitro-culture system with those of field-grown mature grasses (pH 8.5). In spite of the differences in the growing environment and age, the morphological features of the roots under pH 8.5 were identical between the in vitro-grown and field-grown grasses. Here, we propose a simple in vitro-culture method of alkaligrass seedlings under a controlled condition. The alkaligrass seedlings in our in vitro-culture system are compatible to field-grown alkaligrass. Thus, our in vitro system is proved to be useful for further investigations, such as proteomics approach, of this plant.
