METHOD
Protein profiling of Piper sarmentosum
Siang-Yian Shim1, Rasadah Mat Ali2, Lay-Harn Gam1*
1School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia;
2Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor Darul Ehsan, Malaysia.
*Author for Correspondence.
Dr Lay-Harn Gam, School of Pharmaceutical Sciences,
Universiti Sains Malaysia,
11800 USM, Penang, Malaysia.
Tel: 604-6533888 ext 2208,
Fax: 604-6570017,
Email: This email address is being protected from spambots. You need JavaScript enabled to view it..
Abstract.
Piper sarmentosum is a medicinal herb commonly used to treat fever, cough, asthma and pleurisy. In this study, phosphate extraction buffer (ph) was used to extract total cellular proteins from the leaves of P. sarmentosum. Upon sodium do-decyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation, the results showed that most of the extracted proteins migrated in the range of 25 kDa to 75 kDa MW. Each of the bands on the gel was then excised and digested with trypsin and subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS) for protein identification. A series of proteins have been identified from the MS/MS data. The proteins comprised plant defense proteins, structural proteins and enzymes. We have also isolated different isoforms of RuBisCO from the same plants, which were confirmed by mass spectrometry analysis. Amongst the proteins identified was pectinesterase, which is used commercially in various processes including processing of fruits and vegetables.
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