As. Pac. J. Mol. Biol. & Biotech., Jan 2013 Vol. 1, 29-34
In vitro propagation of Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. via asymbiotic seed germination
P. Servin Wesley1, B. Chitra Devi2*, B. Sahaya Shibu1 and Sarmad Moin1
1Department of Biotechnology, Karpagam University, Coimbatore, Tamilnadu, India.
2Department of Botany, Karpagam University, Coimbatore, Tamilnadu, India.
* Author for correspondence: B. Chitra Devi
Assistant Professor, Department of Botany, Karpagam University, Coimbatore, Tamilnadu, India.
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Abstract.
The application of plant tissue culture techniques for conservation and propagation of many threatened species requires an efficient in vitro regeneration protocol. In this study, various basal media were evaluated to determine their effectiveness in promoting asymbiotic seed germination of three important orchid species, Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. Five different basal media, Murashige and Skoog medium (MS), Linsmaier and Skoog medium (LS), Lindemann orchid medium (LOM), Schenk and Hildebrandt medium (SH) and Knudson C medium (KC) were evaluated, among which LS and LOM elicited a better response from all the three orchids. Maximum germination of Coelogyne breviscapa and Flickingeria nodosa seeds was observed on LOM and for Dendrobium aqueum LS medium elicited a better response.
