As. Pac. J. Mol. Biol. & Biotech., Jan 2013 Vol. 1, 19-28
In vitro cucumber haploid line generation in several new cultivars
E. Moqbeli, Gh. Peyvast, Y. Hamidoghli and J.A. Olfati*
Horticultural Department, Faculty of Agriculture, University of Guilan, , Rasht, Iran. I.R
* Author for correspondence: J.A. Olfati
Horticultural Department, Faculty of Agriculture, University of Guilan, Rasht, Iran. I.R.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract.
Due to recent developments, pure lines can now be produced in a short time using in vitro techniques and therefore reduce by several years the time required for conventional plant breeding programmes. In the current study, six F1 cucumber hybrids were investigated, namely ‘Kashmir’, ‘Adergreen’, ‘Summerstar’, ‘Royal’, ‘2010-3’ and ‘502×605’. Unfertilized ovaries were harvested and placed on solid MS medium in Petri dishes. Immediately after placing the unfertilized ovary slices of each variety on induction medium containing different concentrations of Thidiazuron (TDZ) (0, 0.01, 0.02, 0.03 and 0.04 mg∙L-1), they were exposed to a thermal shock pretreatment at 35±1°C for 0, 2, 3 or 4 days. The first visual structures formed after 3 weeks in culture. Cultivar, TDZ concentration and temperature pretreatment all had a significant effect on cucumber embryogenesis. The greatest embryogenesis success was obtained in the ‘summerstar’ variety cultured in medium with 0.04 mg∙L-1 TDZ. TDZ also had a positive effect on embryo formation in our work.
