Functional isocitrate Iyase gene promoter of cucumber on GUS expression in germinating seeds of transgenic tobacco
1. Ismail*1, L.D. Bellis1,2 and S.M. Smith1
1Institute of Cell and Molecular Biology, University of Edinburgh, the King's Buildings,
Mayfield Road, Edinburgh, EH9 3JH, United Kindom;
2Dipartimento di Biologia Piante Agrarie, sez. Fisiologia Vegetale, Universita di Pisa,
via Mariscoglia 34, 56124 Pisa Italy
Received 16 June 2000 / Accepted 14 September 2000
Abstract.
A series of cucumber isocitrate lyase (ICL) gene promoter linked to GUS were introduced into Nicotiana plumbaginifolia by leaf disc transformation using Agrobacterium tumefaciens. The plants were self fertilised and seeds germinated for GUS assay. With 2900 bp ICL promoter, the GUS activity rose from stage 0 to its peak at stage 4 and then fell at stage 7. Deletion to 1568 bp greatly reduced the GUS activity suggesting for a cis acting sequences between the two regions required for seeds germination. Further deletions of ICL promoter retained a low level of GUS activity. Nevertheless, GUS histochemical assay showed that constructs with ICL promoter of 1568 lip, 1367 bp, 1193 and 572 bp directed GUS expression suggesting a qualitative germination response. All of the ICL promoter constructs caused organ specific pattern of GUS expression in cotyledons and expression was also observed in the root hairs of the seedlings.
Keywords: Agrobactetium tumefaciens, isocitrate lyase promoter, GUS, germinating seeds, tobacco
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