Simultaneous detection of CYP2C8*2, CYP2C8*3, CYP2C8*4 and CYP2C8*5 alleles by a nested PCR method.
Wardah Yusof *1, 2 and Siew Hua Gan1
1Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
2Central Research Lab, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
*Author for Correspondence.
Department of Pharmacology,
School of Medical Sciences,
Universiti Sains Malaysia,
Kubang Kerian, Kelantan, Malaysia.
Tel. 609-7664187. Fax. 609-7653370.
Email address: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract.
Cytochrome P450 (CYP) 2C8 plays a significant role in metabolizing many clinically important drugs such as paclitaxel, amiodarone and amodiaquine. A simultaneous detection of CYP2C8*2, CYP2C8*3, CYP2C8*4 and CYP2C8*5 alleles by a PCR method will be useful for detecting single nucleotide polymorphisms (SNPs) on the CYP2C8 gene that may contribute to the inter-individual variability in metabolizing these drugs. This is a blinded randomised cross over study conducted in 24 healthy normal volunteers who received the anti-malarial drug amodiaquine and artesunate both as individual agents and in a fixed combination, over two study phases to determine the safety profile of the two preparations. Genomic DNA was isolated from the volunteers using a DNA extraction kit. A nested PCR method was applied to detect all of the CYP2C8 variants. Regions from exons 3, 5 and 8 of the gene were simultaneously amplified using a first PCR step (PCR1).
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