As. Pac. J. Mol. Biol. & Biotech., January 2010 Vol. 18, 111-114
Effects of Some Cytokinins, Auxins and Medium Constituents on In Vitro Propagation of Polystachya sp.
Chamchuree Sotthikul1,3* Parichat Choomporn2, Siriwan Kammuen1 and Chuntana Suwanthada1,3
1Department of Plant Science and Natural Resources, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. 2H.M.the King’s Initiative Centre for Flower and Fruit Propagation, c/o Chiang Mai University, Thailand. 3Huai Hong Khrai Royal Development Study Centre, Doi Saket, Chiang Mai 52200, Thailand.
*Author for Correspondence. Department of Plant Science and Natural Resources, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
Abstract. A study on in vitro propagation of a wild orchid, Polystachya sp., was carried out by culturing shoots on modified VW (1949) or modified MS (1962) media. It was found that the plants could grow on both modified media supplemented with activated charcoal, 2 mg/L kinetin, or 2 mg/L BAP. MS media with 0.25 mg/L IBA, 0.5-1.0 mg/L NAA promoted root length of 0.40 cm. Combination of 1.0 mg/L NAA and 1.0 mg/L IBA induced 4.10 roots/shoot. The rooting plants could successfully grow in the greenhouse.
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