As. Pac. J. Mol. Biol. & Biotech., Jan 2015 Vol. 1, 20-23
Methods : Manipulation of Benzyl Acetate and Jasmone Content of Jasminum sambac L. Using Modified Murashige and Skoog Medium on Callus Explant
Dwie Retna Suryaningsih*, Sri Arijanti Prakoeswa and Ribkahwati
Faculty of Agriculture, University of Wijaya Kusuma Surabaya, East Java, Indonesia.
* Author for correspondence: Ribkahwati Tanowidjaya
Faculty of Agriculture, University of Wijaya Kusuma Surabaya, East Java, Indonesia.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract.
Jasmine (Jasminum sambac L.) is a flowering plant that grows in shrub form. Jasmine flowers have been extensively used as ornamental and for the production of fragrances, flowers, tea and essential oils. The amount of essential oil that can be collected from jasmine petals is very small relative to the material required, but it is valued as the raw material for natural perfume and aromatherapy treatments. This study attempted to improve propagation from explant to manipulate essential jasmine oil production. Modified Murashige and Skoog (MS) medium, produced from carbohydrate precursor compounds (MS + 20 % fructose, MS + 20 % glucose and MS + 20 % sucrose), was used to produce explants from young leaves and calluses. Essential oil (benzyl acetate and jasmine) content was highest in jasmine explant calluses grown on MS + 20 % sucrose. The content of benzyl acetate reached 1.27 % and jasmone content reached 1.15 % in 12 weeks old calluses.
