As. Pac. J. Mol. Biol. & Biotech., Jun 2017 Vol. 1, 18-30
Genetic diversity among different accessions of maize (Zea Mays L.) determined using microsatellite markers
A. A. Abdel Sattar1, R. M. Esmail1, Sherin A. Mahfouze1*, Heba A. Mahfouze1, M. A. Abou-Ellail2
1National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department, Dokki, 12622, Egypt 2University of Aswan, Faculty of Agriculture and Natural Resources, Department of Genetics , Aswan, Egypt.
* Author for correspondence: Sherin A. Mahfouze
National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department, Dokki, 12622, Egypt.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract.
Maize (Zea mays L.,) is the second most crucial cereal crop after wheat in Egypt and the world. The objective of this study was to evaluate the genetic variability among different accessions of maize by Simple sequence repeat (SSR) analysis and inter simple sequence repeats (ISSR) markers. Six SSR and four ISSR markers were used to assess the genetic variability among 15 maize accessions. A genetic similarity (GS) matrix was produced on the basis of Nei and Li’s coefficients. The coefficients were applied to make clusters using an un-weighted pair group method of arithmetic means (UPGMA) which separated the 15 accessions into three main groups based on SSR and ISSR combination: (I) DTMA-252, DTMA-257, DTMA-261 and DTMA-269; (II) DTMA-52, DTMA-103, DTMA-107, DTMA-125, DTMA-207, DTMA-210, DTMA-216 and DTMA-217; and (III) DTMA-2, DTMA-13 and DTMA-79. GDs among the 15 accessions ranged from 0.67 to 0.89. The maximum genetic distance (GD) value (0.89) was recorded between accessions DTMA-210 and DTMA-216, and the lowest (0.67) between accessions DTMA-2 and DTMA-207. SSR and ISSR techniques were shown to be adequately credible genetic tools for determination of genetic distances among 15 maize accessions.
