As. Pac. J. Mol. Biol. & Biotech., Dec 1998 Vol. 6(2) : 171-174
Stability enhancement of protease by immobilization
A Chellapandian1 and C.A. Sastry2*
1Department of Bioengineering, Institute of Biotechnology, UNAM, Apdo 510 3 Cuernavaca, Morelos 62271, Mexico. 2Institute of Advanced Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
(Received 18 February 1998 / Accepted 26 February 1998)
Abstract. The stability of neutral and alkaline proteases immobilized on hexamethylene diamine coated vermiculite by covalent linkage using bifunctional reagent glutaraldehyde were investigated. Immobilized proteases showed an improved thermal and pH stability when compared to the free enzymes. The stability of the immobilized protease towards urea was studied both in the presence and absence of calcium chloride
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