Properties of four cucumber mosaic cucumovirus isolates from West of Malaysia
O.M. EI Sanousi1, C.A. Ong2, K Yusoff1, S. Napis3 and N. Abdul Sarnad1*
1Dept. of Biochemistry and Microbiology, 3Dept. of Biotechnology,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
2MARDI Serdang, G.P. 0. Box 12301, 50 774 Kuala Lumpur, Malaysia
(Received 30 April 1998 / Accepted 31 October 1998)
Abstract.
Four cucumber mosaic cucumovirus (CMV) isolates from different hosts and localities were differentiated on the basis of biological and serological properties, and polymerase chain reaction (PCR). The first two isolates (CMV-3 and CMV-7) were isolated from tobacco in Telong, Kelantan; the third (CMV-4) was from chilli in MARDI Jalan Kebun, Klang; and the fourth isolate (CMV-6 ) was from purple cleome in Sri Kembangan, Selangor. These isolates could be distinguished from one another by the symptoms produced in several plant species. Aphid transmission tests revealed that all the isolates could be transmitted by Aphis gossypii Glover with higher efficiency than A. craccivora Koch. Immunodiffusion tests revealed that all the isolates were closely related since all the heterologous titres were within the same two fold dilution of each other. Double antibody sandwich enzyme linked immunosorbent assays (DAS-ELISA), revealed that the isolates CMV 3, 6, 7 had a closer relationship to each other than to CMV-4. All the isolates were closely related to D strain (DTL serogroup) but not to Q strain (ToRS serogroup), A single band of about 487 bp was successfully amplified from the coat protein gene of each of the CMV isolates. The PCR product could be digested by Msp I to produce two bands of approximately 337 and 151 bp but could not be digested by EcoR 1. Result of analysis of the biological and serological properties as well as PCR confirmed that all the isolates belonged to subgroup I of CMV.
Keywords: cucumovirus, host range, serology, PCR
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