Allele frequency distribution of the VNTR locus d1s80 and the genetic relationship of Filipinos to other populations
Sheila R. Alcantara' and Saturnina C. Halos'*
'National Institute of Molecular Biology and Biotechnology, College of Science, University of the Philippines
Diliman, Quezon City 1101
2Biology Department, College of Science, De La Salle University, Taft Avenue,
Manila, Philippines
(Received 19 May 19981 Accepted 2 July 1998)
Abstract.
The allele frequency distribution of the D1S80 locus of a Filipino (Davao) population was generated using Amplified Fragment Length Polymorphism (AMP FLP) analysis. Forty two (42) genotypes and 18 alleles ranging from <14 to >>41 repeat units were observed. The most common alleles were 18, 24, 30 and 31 repeats. Three rare alleles were found of which two (<14, >>41) were not previously reported in a Filipino population. This population was in Hardy Weinberg equilibrium with respect to this locus. The locus has an observed heterozygosity of 0.76, gene diversity of 0.86 and a power of discrimination of 0.95, making it a very useful marker for human identification and paternity testing. Using the DISSO allele frequency data, the genetic relationships of the Filipino, Chinese, Korean, Japanese, Caucasians, Hispanics and African Americans were deduced by cluster analysis based on similarities. As expected, Filipinos and the 3 Asian populations Clustered together vis a vis a separate cluster of the Caucasians and Hispanics whereas the African Americans were well separated from these clusters. The analysis also indicated that the Chinese were more similar to the Japanese and the Koreans than they were to the Filipinos.
Keywords: allele frequency, VNTR, d1s80, Filipinos, genetic relationship
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