Studies on the improvement of in vitro shoot regeneration of muskmelon (Cucumis melo L.), cv. Birdie
A.K.M. Mohiuddin1, M. K. U. Chowdhury 2,a, Zaliha C. Abdullah3, K. Harikrishna1 and Suhaimi Napis1*
1GENTECT, Department of Biotechnology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;
2PORIM, P.O. Box 10620, 50720 Kuala Lumpur, Malaysia and 3CRAUN, Land Custody and Development
Authority, Jln. Santubong, Petra Jaya, 93055 Kuching, Sarawak, Malaysia.
(Received 12 December 1997 / Accepted 11 February 1998)
Abstract.
An improved in vitro direct shoot organogenesis plant regeneration system has been developed by culturing proximal cotyledon, distal cotyledon and petiole segments from muskmelon (Cucumis melo, L) cv. Birdie seedlings on MS medium containing BA. BA at 1 and 2 mg-1 was found to improve induction of shoot primordia production significantly from explants of proximal cotyledon, distal cotyledons as well as petiole segments. Shoot primordia was produced after 9-11 days of culture and the elongation of 15 day old shoot primordia was significantly improved when subcultured onto MS medium containing BA at 0.1, 0.3, and 0.5 mgl-1 for prox-1imal cotyledon, petiole and distal cotyledon derived regenerants, respectively. No morphological abnormalities were observed with regenerants produced using these BA concentrations. Significantly improved rooting frequencies of 88%, 80% and 92% were obtained with MS medium containing NAA at 0.01, 0.03 and 0.01 mg1-1, from proximal cotyledon, distal cotyledon and petiole derived shoots respectively. Dark treatment of non rooted regenerants derived from all three explants of muskmelon was sufficient to promote rooting
Keywords: Shoot Regeneration, Muskmelon, Cucumis Melo L.
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