Assessment of a nested PCR for the detection of Chlamydia trachomatis in urine samples
Y,F. Ngeow*, L Rajan, V, Hema and CA Gaydos1
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
The Johns Hopkins University, Chlamydia Research Laboratory, Baltimore, USA.
(Received 29 December 1998 / Accepted 10 January 1998)
Abstract.
A major outer membrane protein (MOMP) based nested polymerase chain reaction (PCR) for the detection of Chlamydia trochomatios was evaluated for urine specimens collected from patients attending sexually trans. mitted disease clinic. A hundred and thirty nine samples were tooted by the nested VCR and a commercially available ligase chain reaction (LCR) ;assay called the LCx from Abbott Laboratories, USA, To resolve discrepant results, 87 of these specimen were also tested by a second LCR amplifying it di (OMPI) gene than the plasmi.d gene targetted in the LCx, The remainder 32 specimens were examined by the IDEIA Chlamydia, an enzyme immunoassay from DAKO Diagnostic@ Ltd,, UK, Using an expanded "gold standard" consisting of two positive test results, the sensitivity, specificity, positive find negative predictive values of the nested VCR were found to be 55.1%, 98,0%, 98,0% and 55.1% respectively. There was no significant difference in the test porformance for male and female urine
Keywords: Chlamydia Trachomatis, Urine, PCR
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