RAPD profile variation amongst cultivated, wild and irradiation derived variants of banana
V.M. Kulkarni1*, S.A. Ranade2,T.R. Ganapathi1, P.Suprasanna1, V.A. Bapat1, K.K. Ussuf1 and P.S. Rao1
1Nuclear Agriculture and Biotechnology Division,
Bhabha Atomic Research Center, Trombay, Mumbai 400 085, India.
'Plant Molecular Biology Division, National Botanical Research Institute, Lucknow 226 001, India
(Received 22 June 1999 / Accepted 190 October l999)
Abstract.
Banana, plantain and cooking banana (all belonging to the genus Musa) are crops of great importance for people in the developing countries and are rich sources of calories, several vitamins and several inorganic minerals. The cultivated bananas are derived from two diploid species, Musa acuminata (genome AA) and Musa balbisiana (genome BB) such that an entire range of triploid and tetraploid forms exist with varying degrees of either or both AA and BB genomes. The classification of banana germplasm has been confounded by the traditional practice of assigning local and vernacular names to the cultivars and varieties. The present study was carried out, in case of 29 varieties collected from different places in India, with the objective of distinguishing between the varieties at the molecular levels. Additionally, 1 genotype belonging to a closely related genus, namely, Ensete superbum was also included as an out group in the studies. The RAPD profiles of these 30 plants enabled distinction between the irradiated and unirradiated variety in an apparently dosage dependent manner. However, the cluster analysis did not group the varieites with any specific trend. Of all the varieties, 30 Gy irradiated Basrai and Williams were most similar to each other (SI=0.79) while the varieties Monthan and Chakkerkeli were the least similar (SI=0.22).
Keywords: Bananas, Musa, Ensete superbum, genetic variation, irradiation derived mutants, RAPD
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