Analysis of fimbrial subunit gene of Dichelobacter nodosus isolated from footrot infected sheep in Malaysia
Z. Zunita1*, A.R. Sheikh Omar1, A. R. Mutalib1, M.L. Mohd Azmi I and Son Radu2
1Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia
2Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia
Received 16 August 2001 / Accepted 8 December 2001
Abstract.
Dichelobacter nodosus serogroup B is the most predominant serogroup in Malaysia and was found to be distinct from the prototype strains. In the present study, we investigate the relationship between fimbriae from different strains isolated in Malaysia and the prototype strains. None of the local isolates had identical sequence with the prototype strain. Isolates exhibited 94 to 97% of amino acid similarities (identities and conserved changes) to D. nodosus prototype strains. Unique sequences for local isolates arc the NQA sequence at AP61, isoleucine at AP67 and threonine at AP 79. There is no substitution in the hypervariable region API 17 124 in all isolates. These results strengthen the suggestion that this region is the common determinant fro the B serogroup. Most importantly, the data in the study is essential for future development of specific vaccine against footrot disease in Malaysia.
Keywords: footrot, Dichelobacter nodosus, sheep, fimbriae
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