Comparision of a dipstick colloidal dye immunoassay with sandwich elisa for the detection of circulating antigens in patients infected with Brugia malayi.
Wan Omar Abdullah*, Sulaiman Osman1, Abdul Manaf Ali2, Yusof Suboh1
and Low Kiat Cheong1
Department of Clinical Laboratory Sciences, Faculty of Medicine and Health Sciences, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor; 1Department of Parasitology and Medical Entomology,
Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz,
50300 Kuala Lumpur1 ; 2Department of Biotechnology, Faculty of Food Sciences and Biotechnology,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor
(Received 10 March 2000 / Accepted 18 February 2001)
Abstract.
The performance of a newly developed dipstick colloidal dye immunoassay (DIA) was compared with that of a previously described Sandwich ELISA to detect parasite antigens in sera from patients with brugian filariasis. The rabbit polyclonal antibodies (RbBmCAg) and the same patients' sera were used in both the tests. In the DIA, 69 of 70 sera from well characterized microfilaremic patients were deemed to contain filarial antigens when screened at a dilution of 1:50. End titres were 1: 10 to 1280. The specificity of both assays was >95% but their sentivity was remarkably different. We have used 6 µl of the RbBmCAg per dipstick in comparision to 150 ?l per well for Sandwich ELISA. The DIA is a rapid test and can be read in approximate 2 h. In addition, coloured dots developed in the DIA can be qualitatively assessed visually for intensity. The DIA does not require sophisticated equipment or radioactivity, and is therefore suitable for field application.
Keywords: Dipstick, Colloidal Dye, Immunoassay, Filariasis
|
