A study on DNA the polymorphic markers in the grouper, Epinephelus merra
Andi Parenrengi1,2* ,Lokman Shamsudin2 , Patimah Ismail3, and Nakisah Mat Amin2
1Research Institute for Coastal Fisheries, Jalan Makmur Daeng Sitakka No. 129, Maros, Sulawesi
Selatan, 90511, Indonesia; 2 Faculty of Science and Technology, Universiti Putra Malaysia Terengganu,
Mengabang Teliput, 21030, Kuala Terengganu, Malaysia; 3Faculty of Medicine and Health Science,
Universiti Putra Malaysia, 43400 Serdang, Selangor D.E, Malaysia.
(Received 16 April 2001 / Accepted 5 July 2001)
Abstract.
Random Amplified Polymorphic DNA (RAPD) technique has been widely applied for genomic mapping, taxonomy/identification, genetic inheritance, sex determination, conservation, population study, and DNA fingerprinting of fish. The grouper, Epinephelus merra collected form Makassar Strait, South Sulawesi, Indonesia was used for the study. Ten RAPD primers (OPA 02, OPA 06, OPA 08, OPA 10, OPA 15, OPA 16, OPA 17, OPA 18, OPA 19, and CA 5) out of twenty four screened primer were selected for further analysis based on the clarity and sharpness of amplification fragment profiles. The ten primers generated a total of 72 loci (fragments) with 41 polymorphic fragments (56.9%). The number of genotypes produced for each primer varied from three to six. The OPA 15 produced the highest polymorphism (85.7%) while OPA 18 produced the lowest polymorphism (33.3%). RAPD bands of E. merra ranged from five to eight and its size range between 200 3000bp. The average of genetic distance between individuals was 0.2737±0.1051.
Keywords: DNA fingerprinting, Epinephelus merra, PCR, polymorphism, RAPD
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