Bioactivity of Selected Actinomycetes Against Ganoderma boninense
Tan, C. J.1, How, K. C.2, Loh-Mia, P. P.1, Ismet, A.1, Getha, K.2, Seki, T.3 and Vikineswary, S.1*
1Institute of Biological Sciences, Faculty of Science, University of Malaya 50603 Kuala Lumpur, Malaysia.
2Institute of Postgraduate Studies,University of Malaya 50603 Kuala Lumpur, Malaysia.
3ICBiotech, Osaka University, Osaka, Japan.
Abstract.
Of 42 strains and 65 crude extracts of actinomycetes studied, 12 positive strains and 22 positive extracts were obtained. The strains were isolated from soil/mud samples of mangrove forest, sandbar litter, oil palm plantation and peat soil. Regardless of whether the test strains were neutrophilic or acido-tolerant, Streptomyces spp were noted to be more active against Ganoderma boninense compared to Micromonospora spp. The percentage of Micromonospora strains with bioactivity against G. boninense in primary screening was found to be significantly lower compared to secondary screening, in which crude extracts were prepared and tested. However, the difference between the percentage of positive Streptomycete strains was found to be small and insignificant between the two screenings. Three patterns of fungal mycelial abnormalities were observed in this preliminary study, which suggested production of antibiotic substances by the actinomycete strains to inhibit growth of G. boninense. The results indicated the possibilities of utilizing actinomycetes as biocontrol agent of oil palm basal stem rot.
Keywords: acido-tolerant actinomycetes, antifungal activity, Ganoderma boninense, Micromonospora spp., Streptomyces spp.
*Author for Correspondence.
Mailing address: Institute of Biological Sciences, Faculty of Science, Universiti Malaya 50603 Kuala Lumpur, Malaysia.
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