Extra dose of Dsn1p (Yir010wp) expressed from episomal plasmid in normal Saccharomyces cerevisiae diploid wild type has augmented growth rate and altered budding pattern, suggesting its growth stimulating property
Yiap, B.C.1, C.M.V.L. Wong1,2, Hirzun, M.Y.3, Raha, A.R.1,2*
1Department of Biotechnology, Faculty of Food Science and Biotechnology,
2Fermentation Technology Unit, Enzyme & Microbial Technology Lab, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
3Sime Darby Technology Center, 2 Jalan Tandang, 46050, Petaling Jaya, Selangor, Malaysia.
*Author for Correspondence.
Dept. of Biotechnology, Fac. of Food Sciences and Biotechnology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor.
Tel: 03-8656 6421 / 8656 6423. Fax: 03-8656 7948 / 8942 3552.
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Key words: Budding, DSN1, gene dosage sensitivity, growth stimulating.
Abstract.
DSN1 is a spindle pole body component that functions in the chromosomal segregation process of yeast. The effect of additional DSN1 gene dosage on the yeast cell has yet to be established. The work from this study indicated that an extra gene dosage of DSN1 caused abnormalities in both cellular growth and budding pattern. The yeast cells carrying an additional copy of DSN1 gene have an increased growth rate and they formed a highly retarded multiple bud complex. These phenomena suggest possible growth stimulating property of the gene product.
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