As. Pac. J. Mol. Biol. & Biotech., Dec 2004 Vol. 12, 39-42
Rapid Production of Lilium auratum Bulbs from Zygotic Embryos
Takuo Furyuya1,3 and Koji Nomura1,2*
1Masters Programs of Biosystem Studies, University of Tsukuba, Tsukuba, Ibaraki 305-8572 2Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki 305-8572 3Present address: Ibaraki Prefectural Sashima Senior High School, Shasima, Ibaraki 306-0501
*Author for Correspondence. Institute of Agriculture and forestry, University of Tsukuba, Ibaraki 305-8572. Tel and Fax: +81-29-8536692; Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract. An in vitro culture system for Lilium auratum Lindl. zygotic embryos was established to accelerate the development of bulblets. High rates of germination were observed on 50 days after pollination (DAP) or later, while the rate of normal growth increased after 70 DAP. Peeling of the seed coats was necessary for rapid in vitro germination, indicating inhibition by the seed coats. We found that embryos obtained from 90 DAP capsules were the best materials for suppressing dormancy. Further development of bulblets was accelerated by culturing them in the presence of 9 % sucrose. In this culture system, the period for development of mature bulblets was reduced from a couple of years to one year after pollination.
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