In Vitro Micropropagation of the aquarium plant Ludwigia repens
Meryem Öztürk1, Khalid Mahmood Khawar2, Hasan Hüseyin Atar1, Cengiz Sancak2 and Sebahattin Özcan2*
1Department of Fisheries, Faculty of Agriculture, University of Ankara, 06110 Diskapi, Ankara, Turkey
2Department of Field Crops, Faculty of Agriculture, University of Ankara, 06110 Diskapi, Ankara, Turkey
*Author for Correspondence.
Department of Field Crops, Faculty of Agriculture, University of Ankara, 06110 Diskapi, Ankara, Turkey.
Tel: +90-312-3179815; Fax: +90-312-3179815;
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Key words: Ludwigia repens, micropropagation, thidiazuron (TDZ), 6-benzylaminopurine, and carry over effect
Abstract.
Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and α-naphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm-3 TDZ and 0.1 mg dm-3 NAA. It was noted that TDZ or BAP had inhibitory effect on shoot elongation, which was overcome by subculturing shoots on half-strength MS media without growth regulators after 4 weeks of culture. This also served as rooting media. Rooted plantlets were finally transferred to aquariums containing fresh water with 100% adaptation.
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