Induction and Expression of Cutinase Activity during Saprophytic Growth of the
Fungal Plant Pathogen, Glomerella cingulata.
Farah Diba Abu Bakar1*, Abdul Munir Abdul Murad1, Aidil Abdul Hamid1, Zulkeflie Zamrod1, Nor Muhammad Mahadi1 and Patrick Sullivan2
1School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia.
2Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand.
*Author for Correspondence.
School of BioSciences and Biotechnology,
Faculty of Science and Technology,
Universiti Kebangsaan Malaysia,
43600 Bangi, Malaysia.
Tel : 03-89215980,
Fax : 03- 89252698
E-mail : This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract.
The fungus Glomerella cingulata damages a wide range of crops in the tropics and subtropics. This fungus produces an extracellular enzyme, cutinase, which has been implicated in the plant disease process. A cutin monomer, 16-hydroxyhexadecanoic acid was used to induce cutinolytic esterase activity during saprophytic growth of G. cingulata. Cutinolytic esterase was induced in cultures grown in cutin monomer but was repressed by glucose. The pattern of cutinolytic activity obtained from the induction and enzyme assays correlates with the results of the Northern blot analyses. The cutinolytic activity and expression from this study demonstrates that the enzyme is tightly regulated by the cutin monomer, 16-hydroxyhexadecanoic acid.
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