Abstract The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract

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As. Pac. J. Mol. Biol. & Biotech., June 2005 Vol. 13, 59-62

The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract

Tau Chuan Ling1,2* and Andrew Lyddiatt1,3

1Biochemical Recovery Group, Department of Chemical Engineering, School of Engineering , University of Birmingham, Edgbaston, Birmingham , B15 2TT, UK
2 Current address: Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
3Current address: Millipore Biopharmaceutical Division, Bioprocessing Ltd, Medomsley Road, Consett DH8 6SZ, United Kingdom.

*Author for Correspondence.
Department of Process and Food Engineering
Faculty of Engineering,
Universiti Putra, Malaysia, 43400 UPM
Serdang, Selangor,Malaysia
Tel: 603 – 89466366; Fax: 603 – 86567123
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Key words: anion exchange, dye-ligand, Cibacron Blue 3GA, fluidised bed adsorption, G3PDH

Abstract.
The comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of intracellular enzyme, glyceraldehydes 3-phosphate dehydrogenase (G3PDH) from unclarified disrupted yeast has been undertaken. The commercially available anion exchanger, Streamline QXL and the kiesleguhr-agarose composite adsorbent, Microsorb K6AX derived with dye-ligand (Cibaron Blue 3GA) were employed in fluidized bed experiments. The adsorbents were evaluated in respect of recovery performance in terms of yield, purity and enzyme specific activity.

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