As. Pac. J. Mol. Biol. & Biotech., Dec 2012 Vol. 20 (4)
In vitro propagation of Wedelia trilobata (L.) using Phormidium subincrustatum extracts: A novel approach
M. Keerthiga1*, S. P. Anand1, V. Nandagopalan1, A. Doss1 and S. R. Senthilkumar2
1PG & Research Department of Botany, National College (Autonomous), Tiruchirappalli - 620 001, Tamil Nadu, South India.
2Department of Botany, St. Joseph’s College (Autonomous), Tiruchirappalli - 620 002, Tamil Nadu, South India.
* Author for correspondence: M. Keerthiga
PG & Research Department of Botany, National College (Autonomous), Tiruchirappalli - 620 001, Tamil Nadu, South India.
Abstract.
Most micropropagation involves the proliferation of callus, shoot and root tissue using MS medium supplemented with commercial growth hormones. While such media have arguably been too successful in terms of multiplication yields, it has become increasingly important to improve productivity and reduce the time taken to multiply commercially important material. The present study reveals the potential effect of extracellular products (EP) and biomass water extracts (BWE) of Phormidium subincrustatum on regeneration of Wedelia trilobata. The growth parameters of plantlets (11cm shoot length and 12 leaves per shoot) were proliferated from the nodal explants when cultured on basal MS media supplemented with 10% cyanobacterial extracts as in the positive control. Initiation of callus growth was observed on the cut surfaces of the leaf sections within 10-15 days of culture with MS medium with BWE, compared to the control. Tremendous increase in shoot length and callus volume over a short period indicates that MS media with added cyanobacterial extracellular product can be used as a better alternative to other chemically synthesized growth regulators in MS media for callus and shoot induction.
