As. Pac. J. Mol. Biol. & Biotech., Dec 2012 Vol. 20 (4)
Analysis of Piper sarmentosum proteome using two dimensional gel electrophoresis and mass spectrometry
Siang-Yian Shim and Lay-Harn Gam*
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
* Author for correspondence: Professor Lay-Harn Gam
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
Tel.: 604-6532208, Fax : 604-6570017, Email : This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract.
P. sarmentosum is a traditionally used medicinal plant. Protein profiling of leaf extracts of P. sarmentosum was performed using two-dimensional electrophoresis (2-DE). The 2-DE patterns showed that most of the protein spots were detected in the mass range of 10-90 kDa on a pH 4-7 IPG strip. A total of 239 protein spots were detected, the spots were excised and digested with trypsin and subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS) for protein identification, from which, 276 proteins were identified. 6.2% of these proteins were categorized as defense and stress-related proteins. Many proteins in the category had been reported to possess antioxidant and anti-pathogen properties
