As. Pac. J. Mol. Biol. & Biotech., July 2012 Vol. 3, 99-106
Characterisation of Aeromonas species isolated from diseased fish using ERIC-RAPD markers
Sujata R.Kamble1*, Sudhir U. Meshram1, Arti S. Shanware2
1P.G.Department. of Microbiology,Rashtrasant Tukadoji Maharaj Nagpur University, L.I.T. Premises, Nagpur, India.
2Rajiv Gandhi Biotechnology Centre, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur-440033, India.
* Author for correspondence: Sujata R. Kamble
P.G. Department of Microbiology, Rashtrasant Tukadoji Maharaj Nagpur University, L.I.T. Premises, Nagpur-440033, India.
E-mail : This email address is being protected from spambots. You need JavaScript enabled to view it.. Tel.: +91-9881084246.
Abstract.
Aeromonas spp. are Gram negative bacteria which are pathogenic to fish, amphibians and also humans. For this study 18 strains of Aeromonas were isolated from healthy or diseased fish and water samples collected from several areas of the Vidharbha region of Maharashtra state, India, for identification and characterization. Aeromonas isolates were characterized by Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR and Random Amplified Polymorphic DNA (RAPD). ERIC and RAPD analysis revealed genetic diversity in the Aeromonas genus as judged by comparing banding patterns. Clusters were formed by the unweighted pair group method with arithmetic (UPGMA) averages applied, which correlate with the genetic information of the species. Construction of phylogenetic dendrograms based on Jaccard’s coefficient revealed that isolates can be divided into different clusters and three-dimensional plots extracted by principal coordinate analysis using UPGMA of Aeromonas spp.
