As. Pac. J. Mol. Biol. & Biotech., April 2012 Vol. 2, 59-64
In vitro callus production and antibacterial activity of Barleria lupulina Lindl
Sarmad Moin1*, Sahaya Shibu Babu1, Arumugam Mahalakshmipriya2
1 Department of Biotechnology. Karpagam University, Coimbatore, India.
2 Department of Biotechnology, Dr. NGP Arts and Science College, Coimbatore, India.
* Author for correspondence: Sarmad Moin
Department of Biotechnology, Karpagam University, Coimbatore-641021, India.
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Abstract.
Callus cultures of Barleria lupulina Lindl were established from leaf explants on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA) and 6-benzylaminopurine (BAP), alone and in various combinations. The maximum number of calli was observed in the combination of IAA and BAP (2.0 mg/l). Calli were extracted with methanol to examine the presence of Phytochemicals and its antibacterial activity. Phytochemical analysis revealed the presence of carbohydrates, cardiac glycoside, glycoside, flavonoids, phenols, tannins and terpenoids. The antibacterial activity of the crude methanol extract of the calli showed activity against the Gram positive bacteria Staphylococcus aureus and Bacillus pumilus. Barleria lupulina Lindl leaf-derived callus extract showed the strongest antibacterial activity, with MIC values of 0.375 mg/l and 0.500 mg/l against Staphylococcus aureus and Bacillus pumilus, respectively.
