As. Pac. J. Mol. Biol. & Biotech., July 2013 Vol. 3, 118-123
Genetic diversity in soybean germplasm identified by RAPD markers
Dhirendra Khare, Aanchal Bisen, Priya Nair and Niraj Tripathi
Seed Technology Centre, Jawaharlal Nehru Agricultural University, Jabalpur, India 482004.
* Author for correspondence: Niraj Tripathi
Seed Technology Centre, Jawaharlal Nehru Agricultural University, Jabalpur, India-482004.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it..
Abstract.
Soybean (Glycine max (L.) Merr.) is a important legume crop in developing countries where it helps to meet the expanding needs for protein, edible oil and calories. RAPD methods were used to investigate the genetic diversity of soybean germplasm. The objectives of this work were to investigate the genetic variation in 38 soybean (Glycine max) lines. Thirty RAPD primers amplified 259 RAPD marker loci. The size of amplified fragments ranged from 200-4000bp. Out of these 259 loci, 253 loci were found to be polymorphic (97.68%).The number of bands produced per primer varied from 5 for OPAI-03 to 11 for OPB-08. The average number of bands per primer was 8.63. The genetic diversity observed was high and allowed the formation of three groups and several subgroups.
