As. Pac. J. Mol. Biol. & Biotech., Apr 2013 Vol. 2, -
In vitro propagation and antioxidant enzymes activities of Elephantopus scaber L.
Jyoti Ranjan Rout and Santi Lata Sahoo*
Biochemistry and Molecular Biology Laboratory, Post Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, Odisha-751004, India.
* Author for correspondence: Prof. Santi Lata Sahoo
Post Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751 004, Odisha, India.
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Abstract.
The seeds of Elephantopus scaber L. were aseptically cultured on half-strength Murashige and Skoog (MS) medium without any plant growth regulator. The germinated seed explants were transferred to callus induction medium fortified with different concentrations (0.25, 0.5, 1.0, 1.5 or 2.0 mg L–1) of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 6-benzylaminopurine (BAP) alone and in combination with kinetin (Kin) (0.25, 0.5, 1.0, 1.5 or 2.0 mg L–1). The maximum callus induction (91.6%) was observed with 1.5 mg L-1 2,4-D + 1.5 mg L–1 Kin. Following callus culture, the proliferated calli were transferred for shoot regeneration. Combination of 2.0 mg L–1 BAP + 1.0 mg L–1 a-naphthaleneacetic acid (NAA) was the most effective for shoot regeneration from callus (13.7 shoot/callus). The elongated shoots rooted in half-strength MS medium supplemented with different concentrations of auxins [NAA, indole-3-acetic acid (IAA) and indole 3-butyric acid (IBA)]. NAA was more suitable for root induction (6.2 shoots/callus) when compared to IAA and IBA (3.5 and 3.7, respectively). The in vitro regenerated plantlets were successfully transferred to the greenhouse for acclimatization. The survival of the plantlets under ex vitro condition was recorded as 77%. Assays of antioxidant activity of in vivo and in vitro grown tissues were evaluated as gross parameters of medicinal efficacy. Antioxidant enzymes like superoxide dismutase (SOD) and guaiacol peroxidase (GPX) were significantly more concentrated in the leaf and root samples of in vitro grown plants whereas the catalase (CAT) concentration was greatest in leaf samples of in vivo plants.
