As. Pac. J. Mol. Biol. & Biotech., Jul 2014 Vol. 3, 219-223
In vitro propagation of Boerhavia diffusa L. (Nyctaginaceae) via nodal and leaf explants
T.P. Ragi and B. Sahaya Shibu*
SIAS-Centre for Scientific Research, SAFI Institute of Advanced Study, Malappuram 673633, India.
* Author for correspondence: B. Sahaya Shibu
Department of Biotechnology, SAFI Institute of Advanced Study, Malapuram-673633, Kerala, India.
Tel.: +91 9789212939, Email: This email address is being protected from spambots. You need JavaScript enabled to view it..
Abstract.
An efficient protocol for in vitro propagation of Boerhavia diffusa L. has been standardized using nodal and leaf explants. Experiments were performed to examine the effect of various growth regulators on callus induction, multiple shoot formation and complete plantlet regeneration. Complete success in callus induction with an average of 3 ± 0.66 shoots were observed on Murashige and Skoog (MS) basal medium supplemented with 2,4-D (9.03 µM). Multiple shoot proliferation (4 ± 0.89) was achieved on MS basal medium supplemented with kinetin (4.64 µM). Well rooted plantlets were hardened and acclimatized in the field and achieved a 65 % survival rate.
