As. Pac. J. Mol. Biol. & Biotech., Apr 2014 Vol. 2, 191-198
Micropropagation of Tecomella undulata (Sm.) Seem. and genetic fidelity testing of in vitro raised plants
Suman Kumari and Narender Singh*
Department of Botany, Kurukshetra University, Kurukshetra-136119, India.
* Author for correspondence: Professor Narender Singh*
Department of Botany, Kurukshetra University, Kurukshetra-136119, India.
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Abstract.
An efficient protocol has been developed for plant regeneration from shoot apex explants of Tecomella undulata. Explants were isolated from 2-3 year old plants, cultured on a shoot induction media, and fortified with different concentrations (0.25, 0.5, 1.0 or 2.0 mg l-1) of auxins (NAA, IAA or 2, 4-D) and cytokinins (BAP or Kinetin). The greatest number of shoots (3.0) was obtained on the medium fortified with BAP (1.0 mg l-1). The greatest root induction response (63.8%) was observed on MS half strength semisolid medium supplemented with 5.0 mg l-1 NAA. The regenerated plantlets were acclimatized and transferred to soil for normal growth under field conditions and 60% survived. Random amplified polymorphic DNA markers were used to analyze the genetic fidelity of these in vitro raised plants. Out of the forty three RAPD decamers screened; only ten primers resulted in two to twelve scorable bands. These ten RAPD primers generated 51 amplicons in total, ranging from 200-2,500 bp in size with an average of 5.1 bands per primer. The amplification products were monomorphic in micropropagated plants and similar to the mother plants, confirming the genetic fidelity of in vitro raised plantlets and corroborating the fact that in vitro multiplication through direct organogenesis is the safest method for multiplying true to type plants
