As. Pac. J. Mol. Biol. & Biotech., Jun 2017 Vol. 1, 2-11
Effect of Carbon Sources and Plant Growth Regulators on Efficient Callus Induction and Regeneration in Barley (Hordeum vulgare L.)
S. M. Shahinul Islam* and Mozidul Haque
Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh
* Author for correspondence: Dr. S. M. Shahinul Islam
Associate Professor, Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.
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Abstract.
An efficient callus induction and regeneration system has been developed for BARI barley-6 during this study. Seed-derived embryos were used as explant sources for callus induction. The highest frequency of callus induction (71.38%) was obtained when 3.5 mg/l 2,4-D was provided to seeds for 4 days prior to culture. The lowest callusing (15.55%) was recorded when the seeds were not pretreated with 2,4-D at all (negative control). Various carbohydrates (sucrose, maltose and D-sorbitol) either singly or in combination were added to three basal media, namely MS, B5 and N6. All media were also supplemented with 1.5 mg/l 2,4-D, 300 mg/l L-proline and 300 mg/l casein hydrolysate for improving callus induction. Different PGRs were used with MS medium and its effect on embryogenic callus induction and their subsequent regeneration was observed. 60 g/l D-sorbitol (T6) gave the highest frequency of primary callus induction (89.16%) in MS medium and 15 g/l maltose with 15 g/l D-sorbitol (T9) gave the lowest frequency (11.33%) in B5 medium. The difference between treatments with different carbohydrate sources and the tested media, with MS, N6 and B5 for callus induction, was significant at the level of P