As. Pac. J. Mol. Biol. & Biotech., January 2012 Vol. 20, issue 1, 11-18
Allergen Protein from Mango (Mangifera indica) Flowers Pollen
Gourango Talukdar1, Ismile Hossain1, Nitai Roy1, Niranjon Kumar Sana1, Syed Rashel Kabir1, Kamal Krishna Biswas1, Narayan Roy1 and Ranajit Kumar Shaha2*
1Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh.
2Faculty of Agro Industry & Natural Resources, University Malaysia Kelantan, Karung Berkunci 36, Pengkalan Cheap, 16100 Kota Bharu, Kelantan, Malaysia.
* Author for correspondence: Ranajit Kumar Shaha
Faculty of Agro Industry & Natural Resources, University Malaysia Kelantan, Karung Berkunci 36, Pengkalan Cheap, 16100 Kota Bharu, Kelantan, Malaysia.
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Abstract.
Plant pollen is one of the most common causes of seasonal allergic disease world wide. In this study Mango flower pollen protein (MFPP) was extracted, filtered, fractioned, purified, and the homogeneity of this allergen protein demonstrated by the presence of a single band on SDS-PAGE. This purified protein showed the pectate lyase (Pel) activity expected of MFPP. The yield of MFPP was 4.10% of the total protein present in the aqueous extract of the pollen, while its allergenic activity was about 44% of the total dialyzable activity, as verified by blood tests. The molecular weight of the extracted protein, estimated by SDS-PAGE, was 27,000 Da and the isoelectric point of the extracted protein was 6.5. Application of this protein to mice produced a change in physical appearance and a rise in the levels of eosinophils and neutrophils level by 2%-5.5% and 34%-77.5%, respectively, relative to the normal levels. Hence the present study demonstrates that extracted protein from Mango flower pollen has allergic effects on animals.