As. Pac. J. Mol. Biol. & Biotech., Jul 2014 Vol. 3, 209-218
Development of an efficient protocol for in vitro germination and enhancing protocorm-like body development in three indigenous orchid species in Bangladesh
Bakul Bhattacharjee and S. M. Shahinul Islam*
Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.
* Author for correspondence: Dr. S. M. Shahinul Islam
Associate Professor, Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.
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Abstract.
An efficient protocol has been developed for in vitro seed germination and for growth and development of protocorm likes bodies (PLBs) from three indigenous orchid species namely Acampe premorsa (Roxb.), Agrostophylum khasianum L. and Phalaenopsis cornorerris. We tested three media and among them PM was found to be the best because of the greatest rate of seed germination (98.20%), PLB development and plant formation. PM supplemented with BAP (1.0 mg/l) and NAA (0.5 mg/l) gave a 78.60% PLB formation rate. The MS with 1.0 mg/l BAP and 0.1 mg/l picloram was the most efficient medium for seedling growth of A. premorsa and A. khasianum. But in the case of P. cornorerris the best seedling growth was observed using medium supplemented with 0.5 mg/l BAP and 0.1 mg/l NAA. The maximum number and length of roots and plantlets were observed in the ½MS medium with 1.5% (w/v) sucrose where no hormones were added. The well-rooted plantlets were successfully acclimatized, planted in pots and transferred to the shade house for establishment. This protocol may be applied for the mass propagation of other orchids for commercial use. We conclude that in vitro seed germination is technique with the highest potential for protocorm formation, multiplication and conservation of indigenous orchids.