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* Entrance Fee of RM20 is chargeable to first time applicants for all membership categories except for student membership.

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Abstract Engineering the dexterity of Bacillus silvestris for Polyhydroxybutyrate production - A thrifty approach

As. Pac. J. Mol. Biol. & Biotech., Apr 2013 Vol. 2, -

Engineering the dexterity of Bacillus silvestris for Polyhydroxybutyrate production - A thrifty approach

Adline Princy S.*, Praveen Krishna V.

Quorum Sensing Laboratory, SASTRA’s Hub for Research & Innovation, School of Chemical and Biotechnology, SASTRA University, Tamil Nadu, India.

* Author for correspondence: Associate Professor Dr. S. Adline Princy
School of Chemical and Biotechnology, SASTRA University, Tirumalaisamudrum-613401, Thanjavur, Tamil Nadu, India.
Tel.: +91 4362 264101, Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Organic polymers that can be efficiently degraded by microorganisms can be referred to as biodegradable compounds. Polyhydroxybutyrates (PHB) are decomposable polymers amassed as carbon storage materials in microbial cells, which can be utilized as a substitute for petrochemical centered plastics that pose a major environmental hazard. However, the most challenging problem in commercializing PHB is the high manufacturing cost because of costly carbon substrate and tedious production processes. Hence there is a requirement for appropriate protocols to explore the properties of unique strains and low-cost substrates. Suitable extraction processes are essential for the economical production of PHB. From this point of view the current study was designed to utilize the distillery waste water as a carbon source for the production of PHB from Bacillus silvestris. The study has demonstrated an improved production of PHB from microorganisms in the presence of distillery wastes as a suitable media. Furthermore, five strains (A, B, C, D and E) were obtained by subjecting the wild type strain to UV irradiation, and their PHB production capabilities were studied. The peak production was observed after the 54th hour of growth in the strain E and its PHB yield was significantly higher than that of the wild type strain.

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Abstract Understanding the requirements and factors necessary for the acceptance of genetically modified mosquitoes as a potential malaria control tool in Ghana: a questionnaire survey

As. Pac. J. Mol. Biol. & Biotech., July 2013 Vol. 3, 76-84

Understanding the requirements and factors necessary for the acceptance of genetically modified mosquitoes as a potential malaria control tool in Ghana: a questionnaire survey

Dziedzom K. de Souza1*, Charles A. Brown2, Collins K. Ahorlu1, Takashi Suzuki1,3

1Noguchi Memorial Institute for Medical Research, University of Ghana, P. O. Box LG 581, Legon, Accra, Ghana.
2School of Allied Health Sciences, University of Ghana, P. O. Box KB 52, Korle-Bu, Accra, Ghana.
3Tokyo Medical and Dental University, Tokyo, Japan.


* Author for correspondence: Dziedzom K. de Souza
Noguchi Memorial Institute for Medical Research, University of Ghana, P. O. Box LG 581, Legon, Accra, Ghana.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Genetically Modified Mosquitoes (GMMs) have been long proposed as a potential tool for malaria control. The challenges posed by current control strategies of bednet usage, Indoor Residual Spraying and larviciding, as well as the development of insecticide resistance to all the major classes of insecticides used against the vectors have led to a renewed interest in the use of GMMs. Despite the achievements in developing GMMs, there are risks, benefits and public acceptance challenges that must be addressed in every country before any field trials and eventual release of the GMMs can be undertaken. While various studies on attitudes to GMM and open releases have been undertaken in some developed countries, this information is missing in Africa and other developing countries. We carried out a pilot questionnaire study, aimed at understanding the needs, requirements and factors necessary for the acceptance of GM mosquitoes as a potential malaria control tool in Ghana. The results showed that while some individuals were open to accepting GMMs despite the perceived risks, the decision to accept them was not influenced by education, age, sex or religion. Public education and stakeholder consultations will be essential in obtaining public consent, before embarking on any malaria control using GMMs.

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Abstract Molecular analysis of intracultivar polymorphism in ‘Peddarasam’ mango (Mangifera indica L.) using microsatellite markers

As. Pac. J. Mol. Biol. & Biotech., July 2013 Vol. 3, 93-110

Molecular analysis of intracultivar polymorphism in ‘Peddarasam’ mango (Mangifera indica L.) using microsatellite markers

Hameedunnisa Begum1*, Medagam Thirupathi Reddy1, Surapaneni Malathi1, Boreddy Purushotham Reddy1, Gonela Narshimulu1, Javaregowda Nagaraju2 and Ebrahimali Abubaker Siddiq3

1Vegetable Research Station, Dr.Y.S.R. Horticultural University (Dr.YSRHU), Rajendranagar, Hyderabad, Andhra Pradesh, 500030, India.
2Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad, Andhra Pradesh, 500001, India.
3Institute of Biotechnology (Formerly Biotechnology Unit), Acharya N.G. Ranga Agricultural University (ANGRAU), Rajendranagar, Hyderabad, Andhra Pradesh, 500030, India.


* Author for correspondence: Hameedunnisa Begum
Principal Scientist (Horticulture), Vegetable Research Station, Dr.Y.S.R. Horticultural University, Rajendranagar, Hyderabad-500030, Andhra Pradesh, India.
Tel. and Fax: 040-24018016, Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Genetic heterogeneity within clones in various clonally propagated fruit crops is assumed to be very limited. ‘Peddarasam’ mango is one of the choicest, juicy cultivars of mango (Mangifera indica L.) in the Indian state of Andhra Pradesh for which genetic homogeneity has never been demonstrated. Fruit and leaf samples of 25 accessions of ‘Peddarasam’ (PR Acc-l to PR Acc-25) covering the three eco-geographical regions (Telangana, Rayalaseema and Coastal Andhra) of the state were collected through an exploration survey during the summer of 2009. Morphological descriptors and microsatellite markers were used to determine the intravarietal polymorphism. Of the 109 mango-specific microsatellites validated, 56 primers amplified 144 polymorphic bands ranging from 100 - 300 bp. The polymorphic information content ranged from 0.03 to 0.82 with an average of 0.50. Jaccard’s similarity coefficient ranged between 0.36 and 1.00 signifying wide amplitude (64%) of intracultivar polymorphism, which is the probable reason for heterogeneity in production and quality of this cultivar across the state. Occasional seed propagation, occurrence of bud sports and de novo variation at the SSR loci are likely to be responsible of the intravarietal polymorphism. Microsatellite analysis revealed high intracultivar polymorphism attesting that ‘Panchadarakalasa’, which is cultivated throughout Andhra Pradesh state, is not a pure clone, a fact which allows the genetic breeding of this cultivar by means of mass selection. SSR-82, SSR-15 and MngSSR-24 produced unique solitary alleles of 130 bp, 245 bp and 150 bp for PR Acc-10, PR Acc-12 and PRAcc-15, respectively, information which will enable better identification of accessions from now on. Microsatellite markers were proven to be useful in assessing intracultivar polymorphism and identifying accessions of ‘Peddarasam’. These results provide evidence that genetic variation among 25 accessions of ‘Peddarasam’ is present and can be further utilized in breeding programs, indicating the potential involvement of Tg-COL9 in flower development of teak.

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Abstract Isolation and expression of CONSTANS-like gene in flowering tissues of Teak (Tectona grandis)

As. Pac. J. Mol. Biol. & Biotech., July 2013 Vol. 3, 85-92

Isolation and expression of CONSTANS-like gene in flowering tissues of Teak (Tectona grandis)

Norlia Basherudin, Mohd Rosli Haron, Norwati Adnan and Norwati Muhammad

Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.

* Author for correspondence: Dr Norlia B. Basherudin
Genetic Unit, Forestry Biotechnology Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
Tel.: 603-62797148, Fax: 603-62804614, Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

CONSTANS (CO) plays an important role in flower development. In flowering pathways, CO integrates the circadian clock and genes controlling the meristem identity. In an attempt to isolate the CO gene from teak, we have isolated the CO-like 9 (COL9) gene which was found to be expressed in early flowering tissues. The gene was isolated using a PCR-based cDNA screening method followed by 5’-rapid amplification with cDNA ends (RACE). The gene encodes a protein which is 64% identical to COL9 in Vitis vinifera, 64% to COL9 of Glycine max and 54% to COL9 of Arabidopsis; the gene was thus named Tg-COL9. Gene expression analysis on the gene using quantitative real time PCR showed a similar pattern of expression to COL9 of Arabidopsis, indicating the potential involvement of Tg-COL9 in flower development of teak.

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Abstract A Reliable Protocol for Micropropagation of Esmeralda clarkei Rchb.f. (Orchidaceae)

As. Pac. J. Mol. Biol. & Biotech., July 2013 Vol. 3, 111-117

A Reliable Protocol for Micropropagation of Esmeralda clarkei Rchb.f. (Orchidaceae)

M.R. Paudel* and B. Pant

Plant Biotechnology and Biochemistry Laboratory, Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.

* Author for correspondence: M.R. Paudel
Plant Biotechnology and Biochemistry Laboratory, Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
Tel.: +9779841700627 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

A reliable protocol for micropropagation of Esmeralda clarkei Rchb.f., an epiphytic, ornamental vandaceous or¬chid was established. Mature seeds obtained from a green healthy capsule were inoculated in MS medium containing different concentrations and combinations of Benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) for established aseptic culture. The cultures were maintained at 25±2°C under a 16/8 hour light/dark photoperiod cycle provided by white fluorescent lamps (Philips, India). Protocorms were developed within 16 weeks; protocorms were differentiated into shoots after 25 weeks of seed culture in MS medium without added plant hormones. Among the different nutrient media tried in this study, BAP (0.5-2.0 mg/l) considerably induced multiple shoot growth (with 11 to 14 shoots growing 2.37-2.87 cm in length) from single protocorm explants and multiple shoots (9-11 shoots growing 3.75-3.87 cm in length) from single shoot section explants after 120 days of culture. More than 3 roots not less than 2 cm long were developed through in vitro rooting of shoots within 90 days of culture on MS medium containing NAA (0.5-1.0 mg/l) and various concentrations of plant hormone. In vitro grown plantlets were successfully acclimatized in hardening medium containing sand, soil and saw dust mixed in a 1:1:1 ratio.

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