As. Pac. J. Mol. Biol. & Biotech., Apr 2013 Vol. 2, -
Engineering the dexterity of Bacillus silvestris for Polyhydroxybutyrate production - A thrifty approach
Adline Princy S.*, Praveen Krishna V.
Quorum Sensing Laboratory, SASTRA’s Hub for Research & Innovation, School of Chemical and Biotechnology, SASTRA University, Tamil Nadu, India.
* Author for correspondence: Associate Professor Dr. S. Adline Princy
School of Chemical and Biotechnology, SASTRA University, Tirumalaisamudrum-613401, Thanjavur, Tamil Nadu, India.
Organic polymers that can be efficiently degraded by microorganisms can be referred to as biodegradable compounds. Polyhydroxybutyrates (PHB) are decomposable polymers amassed as carbon storage materials in microbial cells, which can be utilized as a substitute for petrochemical centered plastics that pose a major environmental hazard. However, the most challenging problem in commercializing PHB is the high manufacturing cost because of costly carbon substrate and tedious production processes. Hence there is a requirement for appropriate protocols to explore the properties of unique strains and low-cost substrates. Suitable extraction processes are essential for the economical production of PHB. From this point of view the current study was designed to utilize the distillery waste water as a carbon source for the production of PHB from Bacillus silvestris. The study has demonstrated an improved production of PHB from microorganisms in the presence of distillery wastes as a suitable media. Furthermore, five strains (A, B, C, D and E) were obtained by subjecting the wild type strain to UV irradiation, and their PHB production capabilities were studied. The peak production was observed after the 54th hour of growth in the strain E and its PHB yield was significantly higher than that of the wild type strain.