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* Entrance Fee of RM20 is chargeable to first time applicants for all membership categories except for student membership.

* Membership is open only to those who qualify under the MPS Constitution and is subjected to approval by the Council.

 

Abstract In vitro propagation of Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. via asymbiotic seed germination

As. Pac. J. Mol. Biol. & Biotech., Jan 2013 Vol. 1, 29-34

In vitro propagation of Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. via asymbiotic seed germination

P. Servin Wesley1, B. Chitra Devi2*, B. Sahaya Shibu1 and Sarmad Moin1

1Department of Biotechnology, Karpagam University, Coimbatore, Tamilnadu, India.
2Department of Botany, Karpagam University, Coimbatore, Tamilnadu, India.


* Author for correspondence: B. Chitra Devi
Assistant Professor, Department of Botany, Karpagam University, Coimbatore, Tamilnadu, India.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

The application of plant tissue culture techniques for conservation and propagation of many threatened species requires an efficient in vitro regeneration protocol. In this study, various basal media were evaluated to determine their effectiveness in promoting asymbiotic seed germination of three important orchid species, Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. Five different basal media, Murashige and Skoog medium (MS), Linsmaier and Skoog medium (LS), Lindemann orchid medium (LOM), Schenk and Hildebrandt medium (SH) and Knudson C medium (KC) were evaluated, among which LS and LOM elicited a better response from all the three orchids. Maximum germination of Coelogyne breviscapa and Flickingeria nodosa seeds was observed on LOM and for Dendrobium aqueum LS medium elicited a better response.

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Abstract Review The Cytochrome P450s

As. Pac. J. Mol. Biol. & Biotech., Apr 2013 Vol. 2, 37-42

Review The Cytochrome P450s

Fatma M.A. El-garj*, Mustafa F.F. Wajidi

School of Distance Education, Universiti Sains Malaysia, 11800 Penang, Malaysia.

* Author for correspondence: Fatma M.A. El-garj
School of Distance Education, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Cytochrome P450 monooxygenases are versatile biocatalysts that incorporate oxygen into an enormous range of molecules. The cytochrome P450 proteins are involved in the catalysis of different reactions and these properties have been used for drug improvement. The protein family also includes compounds producing properties such as resistance to insecticides, and the synthesis of valuable chemicals. In this review, we will discuss various aspects of the structure and function of the cytochrome P450s.

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Abstract Characterization of T2 Tobacco Plants expressing the Hepatitis B gene for Molecular and Morphological Characters

As. Pac. J. Mol. Biol. & Biotech., Apr 2013 Vol. 2, -

Characterization of T2 Tobacco Plants expressing the Hepatitis B gene for Molecular and Morphological Characters

Asha Velayudhan Nair1*, Yogendra Kalenahalli Narasimhamurthy2, Paramanahally Hanumanthegowda Ramanjini Gowda3

1School of Biochemistry, University of Bristol, United Kingdom.
2Plant Science Department, McGill University, Montreal, Quebec, Canada.
3Department of Biotechnology, University of Agricultural Sciences, Bangalore, India.


* Author for correspondence: Asha Velayudhan Nair
School of Biochemistry, Medical Sciences Building, University of Bristol, BS8 1TD, United Kingdom.
Tel.: 004401173312167, Email : This email address is being protected from spambots. You need JavaScript enabled to view it. or This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Hepatitis B virus (HBV) infection remains a worldwide health problem and immunization is the only means to prevent the occurrence of this disease. As the available vaccines are very expensive, there is still need for a less expensive vaccine source especially in most of the developing countries. Plants provide great advantages over other expression systems for the production of recombinant proteins effectively at low cost. In this paper we have shown the stable expression of the hepatitis B surface antigen (HBsAg) in transgenic tobacco plants into the second generation; the current primary focus in plant transformation is the stability of transgene expression. The presence of the HBsAg gene in putative transgenics of the T2 generation of tobacco plants was confirmed by PCR analysis that produced a 900 bp amplification product. Transgenic and untransformed (control) tobacco plants were evaluated for the presence and expression of the hepatitis B surface antigen gene by SDS-PAGE, protein dot blot and ELISA. The T2 generation seeds obtained from the transgenic tobacco plants were tested for germination rate in the presence of kanamycin and were observed to have a segregation ratio of 3:1, indicating Mendelian inheritance. Observations of growth and yield parameters of T2 generation tobacco plants transformed with the HBsAg gene were recorded at regular intervals. It was found that the presence of the foreign gene did not inhibit the growth of the transgenic plants; transgenics showed similar growth rate compared to that of the control plants.

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Abstract Review Reactive Oxygen Species: A link between chronic inflammation and cancer

As. Pac. J. Mol. Biol. & Biotech., Apr 2013 Vol. 2, -

Review Reactive Oxygen Species: A link between chronic inflammation and cancer

Khushtar A. Salman* and Sarah Ashraf

Department of Biochemistry, J.N Medical College, Aligarh Muslim University, Aligarh (U.P) India.

* Author for correspondence: Dr. (Mrs) Khushtar A Salman
Department of Biochemistry, J.N Medical College, AMU, Aligarh 202002 (U.P), India..
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Chronic inflammation is associated with an increase reactive oxygen and nitrogen species, giving rise to epigenetic changes, genomic instability and DNA mutations, which thereby contribute to tumour initiation and progression. Regardless of its origin, 'smouldering' inflammation in the tumour microenvironment has many tumour-promoting effects. It is a component of the tumour microenvironment and is a recognized hallmark of cancer. It aids in the proliferation and survival of malignant cells, promotes angiogenesis and metastasis, subverts adaptive immune responses, and alters responses to hormones and chemotherapeutic agents. Transcription factors (e.g. Nuclear Factor kappa-B and STAT3) modulate the inflammatory response through soluble mediators (cytokines, chemokines) and cellular components (e.g. tumour-associated macrophages) promoting tumorigenesis. Chronic inflammation contribute to about 1 in 4 of all cancer cases. Thus, cancer-related inflammation represents a target for novel diagnostic and therapeutic strategies.

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Abstract In vitro propagation and antioxidant enzymes activities of Elephantopus scaber L.

As. Pac. J. Mol. Biol. & Biotech., Apr 2013 Vol. 2, -

In vitro propagation and antioxidant enzymes activities of Elephantopus scaber L.

Jyoti Ranjan Rout and Santi Lata Sahoo*

Biochemistry and Molecular Biology Laboratory, Post Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, Odisha-751004, India.

* Author for correspondence: Prof. Santi Lata Sahoo
Post Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751 004, Odisha, India.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it..

 

Abstract.

The seeds of Elephantopus scaber L. were aseptically cultured on half-strength Murashige and Skoog (MS) medium without any plant growth regulator. The germinated seed explants were transferred to callus induction medium fortified with different concentrations (0.25, 0.5, 1.0, 1.5 or 2.0 mg L–1) of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 6-benzylaminopurine (BAP) alone and in combination with kinetin (Kin) (0.25, 0.5, 1.0, 1.5 or 2.0 mg L–1). The maximum callus induction (91.6%) was observed with 1.5 mg L-1 2,4-D + 1.5 mg L–1 Kin. Following callus culture, the proliferated calli were transferred for shoot regeneration. Combination of 2.0 mg L–1 BAP + 1.0 mg L–1 a-naphthaleneacetic acid (NAA) was the most effective for shoot regeneration from callus (13.7 shoot/callus). The elongated shoots rooted in half-strength MS medium supplemented with different concentrations of auxins [NAA, indole-3-acetic acid (IAA) and indole 3-butyric acid (IBA)]. NAA was more suitable for root induction (6.2 shoots/callus) when compared to IAA and IBA (3.5 and 3.7, respectively). The in vitro regenerated plantlets were successfully transferred to the greenhouse for acclimatization. The survival of the plantlets under ex vitro condition was recorded as 77%. Assays of antioxidant activity of in vivo and in vitro grown tissues were evaluated as gross parameters of medicinal efficacy. Antioxidant enzymes like superoxide dismutase (SOD) and guaiacol peroxidase (GPX) were significantly more concentrated in the leaf and root samples of in vitro grown plants whereas the catalase (CAT) concentration was greatest in leaf samples of in vivo plants.

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4th International Conference on Molecular Biology & Biotechnology

in conjunction with the 27th  Scientific Meeting of the Malaysian Society of Molecular Biology & Biotechnology (MSMBB) 

Beyond 2021: Building Sustainable Science

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