Membership Subscription Rates

 

  • Entrance Fee
  • RM 20
  • For first time applicants only
  • * Entrance Fee of RM20 is chargeable to first time applicants for all membership categories except for student membership.
  • Ordinary Membership
  • RM 40
  • For non-students

 

  • Life Membership
  • RM 400
  • One-off fee for lifetime membership, no renewals required
  • Corporate Membership
  • RM 500
  • For organizations or commercial companies interested in contributing financially to the Society

 

* Entrance Fee of RM20 is chargeable to first time applicants for all membership categories except for student membership.

* Membership is open only to those who qualify under the MPS Constitution and is subjected to approval by the Council.

 

Abstract Methods Paper Optimization of genomic DNA shearing by sonication for next-generation sequencing library preparation

As. Pac. J. Mol. Biol. & Biotech., Jul 2014 Vol. 3, 200-208

Methods Paper Optimization of genomic DNA shearing by sonication for next-generation sequencing library preparation

Le Jie Lee1,2 and Maha Abdullah1*

1Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia,
2Malaysia Genome Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor Malaysia.


* Author for correspondence: Dr. Maha Abdullah
Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it. Tel.: 603-89472375 Fax: 603-89412787,

 

Abstract.

Next-generation sequencing (NGS) technologies, capable of sequencing genomic DNA and RNA at high throughput with unprecedented speed, have revolutionized genomic research as well as clinical diagnosis. DNA fragmentation is a critical step in library preparation in all NGS platforms, and determines the quality and diversity of the final library. DNA shearing by acoustic sonication is one of the ways to randomly break DNA into small fragments, however many variables affect the outcome. Here, we describe an optimized procedure to shear genomic DNA into fragments of 150 bp to 120 bp using a focused-ultrasonicator. Parameters that were assessed included DNA quantity, the effect of repeat shearing, treatment time, peak incident power and shearing reproducibility. This input of pure and optimum quality DNA samples is an essential starting point to the NGS system. We identified peak incident power as being the key determining factor in obtaining small target fragments. By increasing the peak incident power to 75W, a peak size within the 150 bp to 200 bp range was achievable, a result which was reproducible in multiple samples. Repeat shearing and increased treatment time were less successful in producing optimally sized DNA fragments. The proposed method may be used as a guide for NGS users involved in library construction, particularly when small fragment sizes are required.

[Get pdf]

Abstract Development of an efficient protocol for in vitro germination and enhancing protocorm-like body development in three indigenous orchid species in Bangladesh

As. Pac. J. Mol. Biol. & Biotech., Jul 2014 Vol. 3, 209-218

Development of an efficient protocol for in vitro germination and enhancing protocorm-like body development in three indigenous orchid species in Bangladesh

Bakul Bhattacharjee and S. M. Shahinul Islam*

Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.

* Author for correspondence: Dr. S. M. Shahinul Islam
Associate Professor, Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it.. Tel.: +88-0721-750928, Fax: +88-0721-750064, Mobile: +88-01715209907

 

Abstract.

An efficient protocol has been developed for in vitro seed germination and for growth and development of protocorm likes bodies (PLBs) from three indigenous orchid species namely Acampe premorsa (Roxb.), Agrostophylum khasianum L. and Phalaenopsis cornorerris. We tested three media and among them PM was found to be the best because of the greatest rate of seed germination (98.20%), PLB development and plant formation. PM supplemented with BAP (1.0 mg/l) and NAA (0.5 mg/l) gave a 78.60% PLB formation rate. The MS with 1.0 mg/l BAP and 0.1 mg/l picloram was the most efficient medium for seedling growth of A. premorsa and A. khasianum. But in the case of P. cornorerris the best seedling growth was observed using medium supplemented with 0.5 mg/l BAP and 0.1 mg/l NAA. The maximum number and length of roots and plantlets were observed in the ½MS medium with 1.5% (w/v) sucrose where no hormones were added. The well-rooted plantlets were successfully acclimatized, planted in pots and transferred to the shade house for establishment. This protocol may be applied for the mass propagation of other orchids for commercial use. We conclude that in vitro seed germination is technique with the highest potential for protocorm formation, multiplication and conservation of indigenous orchids.

[Get pdf]

Abstract Genetic diversity in white jabon (Anthocephalus cadamba (Roxb.) Miq.) based on AFLP markers

As. Pac. J. Mol. Biol. & Biotech., Jul 2014 Vol. 3, 224-231

Genetic diversity in white jabon (Anthocephalus cadamba (Roxb.) Miq.) based on AFLP markers

Dede J. Sudrajat1,2*, Iskandar Z. Siregar2*, Nurul Khumaida3, Ulfah J. Siregar2,4, Irdika Mansur2,4

1Forest Tree Seed Technology Researach Institute, Bogor, Indonesia.
2Departement of Silviculture-Bogor Agricultural University, Bogor, Indonesia.
3Departemen of Agronomy-Bogor Agricultural University, Bogor, Indonesia.
4SEAMEO-BIOTROP, Bogor, Indonesia.


* Author for correspondence: Dede J. Sudrajat
Forest Tree Seed Technology Researach Institute, Bogor, Indonesian Departement of Silviculture-Bogor Agricultural University, Bogor, Indonesia.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 and

Iskandar Z. Siregar
Departement of Silviculture-Bogor Agricultural University, Bogor, Indonesia.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it..  

 

Abstract.

An amplified fragment length polymorphisms (AFLP) technique was applied to estimate the genetic diversity in four populations of white jabon (Anthocephalus cadamba Miq.) from different islands of Indonesia. The highest genetic diversity was detected in the Kapuas population (He = 0.3339, I = 0.4894), followed by Pomalaa (He = 0.2270, I = 0.3394) and Nusa Kambangan populations (He = 0.1940, I = 0.2904), while the lowest genetic diversity was measured in the Kampar population (He = 0.1489, I = 0.2278). Analysis of population structure revealed that genetic diversity within populations (Hs = 0.2260) represented 73% of the total genetic diversity (Ht = 0.3098). The proportion of the total genetic diversity that was attributed to the population differentiation was high (Gst =0.2707). The geographic distances are not significantly correlated with the genetic distance between the populations of white jabon studied. Cluster analysis placed most accessions from the Kapuas population in a separate cluster from the accessions from the other populations. We conclude that the genetic variability of the studied white jabon accessions is structured according to the origin and the population should be further sampled to increase accuracy of measurements of the genetic diversity.

[Get pdf]

Abstract In vitro propagation of Boerhavia diffusa L. (Nyctaginaceae) via nodal and leaf explants

As. Pac. J. Mol. Biol. & Biotech., Jul 2014 Vol. 3, 219-223

In vitro propagation of Boerhavia diffusa L. (Nyctaginaceae) via nodal and leaf explants

T.P. Ragi and B. Sahaya Shibu*

SIAS-Centre for Scientific Research, SAFI Institute of Advanced Study, Malappuram 673633, India.

* Author for correspondence: B. Sahaya Shibu
Department of Biotechnology, SAFI Institute of Advanced Study, Malapuram-673633, Kerala, India.
Tel.: +91 9789212939, Email: This email address is being protected from spambots. You need JavaScript enabled to view it..

 

Abstract.

An efficient protocol for in vitro propagation of Boerhavia diffusa L. has been standardized using nodal and leaf explants. Experiments were performed to examine the effect of various growth regulators on callus induction, multiple shoot formation and complete plantlet regeneration. Complete success in callus induction with an average of 3 ± 0.66 shoots were observed on Murashige and Skoog (MS) basal medium supplemented with 2,4-D (9.03 µM). Multiple shoot proliferation (4 ± 0.89) was achieved on MS basal medium supplemented with kinetin (4.64 µM). Well rooted plantlets were hardened and acclimatized in the field and achieved a 65 % survival rate.

[Get pdf]

Abstract In vitro cucumber haploid line generation in several new cultivars

As. Pac. J. Mol. Biol. & Biotech., Jan 2013 Vol. 1, 19-28

In vitro cucumber haploid line generation in several new cultivars

E. Moqbeli, Gh. Peyvast, Y. Hamidoghli and J.A. Olfati*

Horticultural Department, Faculty of Agriculture, University of Guilan, , Rasht, Iran. I.R

* Author for correspondence: J.A. Olfati
Horticultural Department, Faculty of Agriculture, University of Guilan, Rasht, Iran. I.R.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Due to recent developments, pure lines can now be produced in a short time using in vitro techniques and therefore reduce by several years the time required for conventional plant breeding programmes. In the current study, six F1 cucumber hybrids were investigated, namely ‘Kashmir’, ‘Adergreen’, ‘Summerstar’, ‘Royal’, ‘2010-3’ and ‘502×605’. Unfertilized ovaries were harvested and placed on solid MS medium in Petri dishes. Immediately after placing the unfertilized ovary slices of each variety on induction medium containing different concentrations of Thidiazuron (TDZ) (0, 0.01, 0.02, 0.03 and 0.04 mg∙L-1), they were exposed to a thermal shock pretreatment at 35±1°C for 0, 2, 3 or 4 days. The first visual structures formed after 3 weeks in culture. Cultivar, TDZ concentration and temperature pretreatment all had a significant effect on cucumber embryogenesis. The greatest embryogenesis success was obtained in the ‘summerstar’ variety cultured in medium with 0.04 mg∙L-1 TDZ. TDZ also had a positive effect on embryo formation in our work.

[Get pdf]

About MSMBB

We are a non-profit organisation that was established in 1988 to promote molecular biology and biotechnology.

Stay Connected on:

    

Next Event

4th International Conference on Molecular Biology & Biotechnology

in conjunction with the 27th  Scientific Meeting of the Malaysian Society of Molecular Biology & Biotechnology (MSMBB) 

Beyond 2021: Building Sustainable Science

1-3rd June2021

Online Conference

Visit

https://themsmbboffice.wixsite.com/4thicmbb2021

 

 

Contacts Us

For general information about MSMBB, including registration, please contact us at:

  Department of Parasitology,
Faculty of Medicine,
University of Malaya,
50603 Kuala Lumpur,
Malaysia.
  This email address is being protected from spambots. You need JavaScript enabled to view it.
  +603 - 7967 4749
  +603 - 7967 4749