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* Entrance Fee of RM20 is chargeable to first time applicants for all membership categories except for student membership.

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Abstract Sample preservation procedures and simple DNA isolation protocols for the tuberous crop, cassava (Manihot esculenta Crantz.)

As. Pac. J. Mol. Biol. & Biotech., Jan 2014 Vol. 1, 164-170

Sample preservation procedures and simple DNA isolation protocols for the tuberous crop, cassava (Manihot esculenta Crantz.)

Nuri Kiptantiyawati1, Nawar Lina Syarifah1, Pradita Maulia2, Etik Sulistiyowati1, Mirza Ramadhana Putra1, Yudiansyah1, Nurul Khumaida1, Sintho Wahyuning Ardie1*

1Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University (IPB), Jl. Meranti Kampus IPB Darmaga 16680.
2Department of Biochemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Jl. Agatis Kampus IPB Darmaga 16680.


* Author for correspondence: Sintho Wahyuning Ardie
Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University (IPB), Jl. Meranti, Kampus IPB Darmaga, Bogor, West Java, Indonesia 16680.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it. Tel.: +62-251-86629353 Fax: +62-251-86629353

 

Abstract.

Large scale collection of samples from remote areas followed by efficient DNA isolation is often required for genetic studies of a crop species using molecular methods. In this paper, we describe the comparison of different tissue preservation procedures, and develop a reliable and efficient protocol for isolating genomic DNA from four cassava (Manihot esculenta Crantz.) genotypes. We found that cassava leaf tissue can be preserved at 4°C for one week without significant loss in yield or quality. Comparison of six DNA isolation protocols revealed that a simplified version of an existing protocol using CTAB buffer was the best method to isolate DNA from leaf samples of ‘Ratim’, ‘Jame-jame’, ‘Malang-4’ and ‘Gajah’ genotypes. The methodology not only obtained high DNA yields of 771, 585, 542 and 2,672 μg DNA/g of sample, respectively, but also produced high quality DNA with A260/A280 ratios of 1.9, 1.9, 1.7 and 2.0, respectively. PCR amplification using the resulting DNA produced reliable and reproducible results, indicating the suitability of DNA for subsequent PCR analyses. This simplified protocol using CTAB buffer removes the necessity for grinding using liquid nitrogen and the use of phenol, PVP (polyvinyl polypyrrolidone), and β-mercaptoethanol in the isolation step, and can thus be considered technically easy and cost-effective.

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Abstract Review Reciprocal Regulation of the Periodontitis and Diabetes

As. Pac. J. Mol. Biol. & Biotech., Apr 2014 Vol. 2, 172-179

Review Reciprocal Regulation of the Periodontitis and Diabetes

Gurmeet Kaur and Adline Princy S*

Quorum Sensing Laboratory, Centre for Research on Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA’s Hub for Research and Innovation (SHRI), SASTRA University, Thanjavur, Tamil Nadu, India.

* Author for correspondence: Associate Professor Dr. Adline Princy S.
Quorum Sensing Laboratory, Centre for Research on Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur – 613 401 Tamil Nadu, India.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it. Tel.: +91 - (4362) 264101 – 108 and +91-9486910054

 

Abstract.

Periodontitis and its association with systemic diseases such as diabetes has been an area of focus recently. Diabetes associated with periodontal pathogens can result in the periodontal tissue damage and hence, resulting in periodontal disease. In the same way, periodontal disease is also involved in the worsening of various systemic diseases through inflammation process. Inflammation due to microbial flora of the oral cavity and infection due to it plays a central role in the development and the interrelation between the two diseases. The inflammatory markers which are responsible for the development of insulin resistance are high in case of patients with periodontitis and thus they are on an edge of developing insulin resistance and further worsening of it. This paper is intended to provide information regarding the role of periodontal disease in systemic diseases and bidirectional relationship between periodontitis and diabetes.

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Abstract A simple in vitro method to study alkaline tolerance in alkaligrass (Puccinellia airoides) at high pH

As. Pac. J. Mol. Biol. & Biotech., Apr 2014 Vol. 2, 185-190

A simple in vitro method to study alkaline tolerance in alkaligrass (Puccinellia airoides) at high pH

Xue Zhang1, Koji Nomura*1, Katsuyoshi Shimizu2 and Ke-Zhang Xu3

1Graduate School of Life and Environmental Science, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan,
2Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan,
3Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, Jilin Province, China.


* Author for correspondence: Koji Nomura
Graduate School of Life and Environmental Science, University of Tsukuba Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it. Tel.: +81-29-853-6692 Fax: +81-29-853-6692

 

Abstract.

Species belonging to genus Puccinellia are commonly called 'alkaligrass' because of their tolerance to saline-alkali environment. Although phyto-remediation of saline-alkali lands by planting alkaligrass is already achieved, its mechanisms of alkali tolerance need to be further comprehensively and thoroughly investigated. In a previous work, we revealed that alkaligrass had high tolerance to a high pH condition already during its germination and initial growth by growing seedlings in vitro. For further studies of alkali tolerance, an in vitro-culture system under a sterile condition is required. In a previous work, we cultured young alkaligrass seedlings on an agar medium to reveal their responses to a high pH stress. In the present work, we elaborated the in vitro method further and developed a convenient, efficient and reliable culture system. We compared the roots of the seedlings that were grown in the in vitro-culture system with those of field-grown mature grasses (pH 8.5). In spite of the differences in the growing environment and age, the morphological features of the roots under pH 8.5 were identical between the in vitro-grown and field-grown grasses. Here, we propose a simple in vitro-culture method of alkaligrass seedlings under a controlled condition. The alkaligrass seedlings in our in vitro-culture system are compatible to field-grown alkaligrass. Thus, our in vitro system is proved to be useful for further investigations, such as proteomics approach, of this plant.

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Abstract Mini Review : Alpha 1-antitrypsin and its involvement in rheumatoid arthritis

As. Pac. J. Mol. Biol. & Biotech., Apr 2014 Vol. 2, 180-184

Mini Review : Alpha 1-antitrypsin and its involvement in rheumatoid arthritis

Khushtar A. Salman*, Akif Ahsan and Sarah Ashraf

Department of Biochemistry, J.N Medical College, Aligarh Muslim University, Aligarh (U.P), India.

* Author for correspondence: Associate Professor Dr. (Mrs) Khushtar A Salman
Department of Biochemistry, J.N Medical College, AMU, Aligarh 202002 (U.P), India.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it..

 

Abstract.

Rheumatoid arthritis is a common autoimmune disorder, most often affecting women from 40 to 60 years old. The major symptom is chronic inflammation of the joints, although the hematologic, cardiovascular, and respiratory systems are also frequently affected. Many individuals with rheumatoid arthritis produce a group of auto-antibodies, known as rheumatoid factors, that are reactive with determinants in the Fc region of IgG. Human alpha 1-antitrypsin is well known as a serine proteinase inhibitor which inhibits proteinase 3, neutrophil elastase, and cathepsin G. These serine proteases are released by joint-invading neutrophils following inflammatory stimuli and have been shown to be involved in development of arthritis. A lack of alpha 1-antitrypsin in patients with rheumatoid arthritis could cause an increase in inflammation because of uninhibited lysosomal enzymes. Future studies will focus on improvement of the therapeutic application by optimising the dose and timing of hAAT or rAAV8 vector delivery, and by development of combination therapy with other anti-arthritic drugs.

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Abstract Micropropagation of Tecomella undulata (Sm.) Seem. and genetic fidelity testing of in vitro raised plants

As. Pac. J. Mol. Biol. & Biotech., Apr 2014 Vol. 2, 191-198

Micropropagation of Tecomella undulata (Sm.) Seem. and genetic fidelity testing of in vitro raised plants

Suman Kumari and Narender Singh*

Department of Botany, Kurukshetra University, Kurukshetra-136119, India.

* Author for correspondence: Professor Narender Singh*
Department of Botany, Kurukshetra University, Kurukshetra-136119, India.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

An efficient protocol has been developed for plant regeneration from shoot apex explants of Tecomella undulata. Explants were isolated from 2-3 year old plants, cultured on a shoot induction media, and fortified with different concentrations (0.25, 0.5, 1.0 or 2.0 mg l-1) of auxins (NAA, IAA or 2, 4-D) and cytokinins (BAP or Kinetin). The greatest number of shoots (3.0) was obtained on the medium fortified with BAP (1.0 mg l-1). The greatest root induction response (63.8%) was observed on MS half strength semisolid medium supplemented with 5.0 mg l-1 NAA. The regenerated plantlets were acclimatized and transferred to soil for normal growth under field conditions and 60% survived. Random amplified polymorphic DNA markers were used to analyze the genetic fidelity of these in vitro raised plants. Out of the forty three RAPD decamers screened; only ten primers resulted in two to twelve scorable bands. These ten RAPD primers generated 51 amplicons in total, ranging from 200-2,500 bp in size with an average of 5.1 bands per primer. The amplification products were monomorphic in micropropagated plants and similar to the mother plants, confirming the genetic fidelity of in vitro raised plantlets and corroborating the fact that in vitro multiplication through direct organogenesis is the safest method for multiplying true to type plants

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