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* Entrance Fee of RM20 is chargeable to first time applicants for all membership categories except for student membership.

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Abstract Watering and nitrogen and potassium fertilization: The significance of abiotic control on Gynura procumbens (Lour.) Merr herbs in Malaysia for better growth and secondary metabolite enrichment

As. Pac. J. Mol. Biol. & Biotech., Apr 2015 Vol. 2, 71-81

Watering and nitrogen and potassium fertilization: The significance of abiotic control on Gynura procumbens (Lour.) Merr herbs in Malaysia for better growth and secondary metabolite enrichment

Mohamad Fhaizal Mohamad Bukhori1,2*, Hawa Z.E. Jaafar1, Ali Ghasemzadeh1

1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
2Centre for Pre-University Studies, Universiti Malaysia Sarawak, 94300 Samarahan, Sarawak, Malaysia.


* Author for correspondence: Mohamad Fhaizal Mohamad Bukhori
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Environmental changes have led to cellular adjustment and adaptation in plant growth. External factors have, for example, influenced the growth pattern of Gynura procumbens plants and led to production of specific secondary metabolite internally for the purpose of differentiation and conditional interaction. These developmental patterns and production of metabolites are expressional characteristics of the plant, and so growers can have only a restricted range of movement or limited control over their reaction to environmental changes compared to their reaction to human or animal interactions. Even though metabolite production is pervasive among the plants, the need to explore abiotic control strategies for regulating the patterns of growth of Gynura procumbens as well as their accumulation of metabolites has been shown to be significant in recent studies of plant-abiotic interactions.

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Abstract Application of cold pretreatment and optimisation of media for enhancement of anther culture response in two barley (Hordeum vulgare L.) genotypes derived from Bangladesh

As. Pac. J. Mol. Biol. & Biotech., Jan 2014 Vol. 1, 127-136

Application of cold pretreatment and optimisation of media for enhancement of anther culture response in two barley (Hordeum vulgare L.) genotypes derived from Bangladesh

Mozidul Haque and S. M. Shahinul Islam*

Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.

* Author for correspondence: Dr. S. M. Shahinul Islam
Associate Professor, Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Rapid development of embryoids and the efficiency of regeneration by anther culture was observed in two barley genotypes. Five semi-solid media viz. MS, B5, N6, FHG and AMS3 were used for embryo induction as well as plant regeneration. Harvested spikes were pretreated with cold for 2, 4, 6, 8, 10, 12, 14 or 16 days at 4°C in the dark. Cold pretreatment for 8-12 days produced the highest frequency of embryoid induction on FHG medium. The FHG medium was modified by MS that was supplemented with 2,4-D (2.0 mg/l), kinetin (0.5 mg/l) and an amino acid (730 mg/l L-glutamine). Out of two genotypes, BB-6 performed better in terms of embryo formation and green plant regeneration in MSR medium. The highest level of embryoid induction (14.6%) was observed at T5 (10 days), producing a total of 13.8% regenerated plantlets and 10.72% green plants in BARI barley-6. Analysis of variance (ANOVA) showed significant differences in embryoids induction rate depending on the duration of cold pretreatment, culture media and between the genotypes. It was observed that the interactions of medium and cold pretreatment, and genotype and medium, were significant in determining the rate of embryoid induction. Cold pretreatment for 10 days of excised anthers from spikes produced optimum results in both genotypes, and the FHG medium was the best out of the five tested for embryoid induction and plant regeneration.

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Abstract Organogenesis from chrysanthemum Dendranthema x grandiflora (Ramat.) Kitamura petals (disc and ray florets) induced by plant growth regulators

As. Pac. J. Mol. Biol. & Biotech., Jan 2014 Vol. 1, 145-151

Organogenesis from chrysanthemum Dendranthema x grandiflora (Ramat.) Kitamura petals (disc and ray florets) induced by plant growth regulators

Jaime A. Teixeira da Silva1,2

1Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan.
2Current address: Miki-cho post office, Ikenobe 3011-2, P. O. Box 7, Kagawa-ken, 761-0799, Japan.


* Author for correspondence: Jaime A. Teixeira da Silva
Miki-cho post office, Ikenobe 3011-2, P. O. Box 7, Kagawa-ken, 761-0799, Japan.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

The chrysanthemum (Dendranthema x grandiflora (Ramat.) Kitamura) is an important ornamental plant. The tissue culture of chrysanthemum is well explored, but the use of petal tissue in regeneration is less undertaken, most likely due to the risk of chimerism. This study provides a way to induce organogenesis (of callus, shoots, and roots) from the petals (disc and ray flowers) of a Japanese cultivar, ‘Shuhou-no-chikara’. The response to a wide range of plant growth regulators (PGRs), under light and dark conditions, and on a Murashige and Skoog basal medium, indicates that trial and error is necessary for the optimisation of a flower-based regeneration protocol. In this study, three trends were observed: 1) There was no difference between the response in the light and the dark, except for colour, or between disc and ray florets; 2) Twelve out of 30 PGRs, when tested alone, provided some organogenic response while the remaining 18 PGRs resulted in no or <5% explant response; and 3) Organogenesis is reported for the first time in chrysanthemum flower tissue for 5 PGRs: adenine sulphate, 2iP, picloram, coconut water (CW), and phloroglucinol (PG). Ads, 6-benzyladenine (BA) and Kin resulted in shoot formation, 2iP, picloram, 2,4-D and TDZ induced callus formation, CW formed shoots and calluses, while PG, IAA, IBA and NAA formed roots, each effective at different concentrations. In the presence of 2 mg L-1 TDZ, 621 mg of callus (fresh weight-basis) formed relative to 76 mg in the control (PGR-free basal medium) while 100% explant survival was observed in the presence of 2 or 4 mg L-1 BA (relative to 5% in the control). Use of petals allows a wider range of explants to be used when other parts of the plant may be recalcitrant, although material is limited to the flowering period unless in vitro flowering can be induced. This data set serves as a preliminary assessment of the response of floral tissue to a wider range of PGRs, applied alone, and serves as a spring-board for further studies that should examine a wider range of cultivars and PGR combinations. The ploidy, genetic stability and success of acclimatisation of such material should also be examined.

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Abstract Effect of silver nitrate and gibberellic acid on in vitro regeneration, flower induction and fruit development in Naga Chilli

As. Pac. J. Mol. Biol. & Biotech., Jan 2014 Vol. 1, 137-144

Effect of silver nitrate and gibberellic acid on in vitro regeneration, flower induction and fruit development in Naga Chilli

G. Bora1,2*, H. K. Gogoi1, P. J. Handique2

1Division of Biotechnology, Defence Research Laboratory, Tezpur-784001, Assam, India.
2Department of Biotechnology, Gauhati University, Guwahati-781014, India.


* Author for correspondence: Ms. Geetashree Bora
Christian Colony, Jail Road, Borbheta, Jorhat-785004, Assam, India.
Tel : 91-7896851882 Email : This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

In vitro flower induction and fruit development are rarely achieved in the genus Capsicum. This communication reports for the first time the induction of in vitro flower induction and fruit development in Bhot jolokia or Naga Chilli under the influence of silver nitrate (AgNO3) and gibberellic acid (GA3). The greatest number of multiple shoots from a single shoot apical meristem was induced in MS medium fortified with BAP (45µML-1), NAA (5.5µML-1) and AgNO3 (35µML-1). Half strength MS medium supplemented with AgNO3 at a concentration of 34µML-1 and GA3 at 28µML-1 was found to be the optimum concentration for in vitro flower induction, and AgNO3 at 32µML-1 added to half strength MS was found best for in vitro fruit development. In vitro regenerated shoots were provided with rooting medium containing MS medium, IBA and BAP at 3.5μML-1 and 2.5μML-1, respectively. Healthy rooted plantlets were transferred to potting substrate where half strength MS medium was enhanced with soil and vermicompost in equal volumes (1:1) for hardening in the poly house. 72.6% of all acclimatised plants were successfully transferred to the open field.

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Abstract In vitro morphogenesis and RAPD analysis of Justicia tranquebariensis L.f.-an important medicinal plant

As. Pac. J. Mol. Biol. & Biotech., Jan 2014 Vol. 1, 152-163

In vitro morphogenesis and RAPD analysis of Justicia tranquebariensis L.f.-an important medicinal plant

S. Raji1, M. Ayyanar2, P. Ponmanickam1 and T. Rajagopal1,3

1Post Graduate Department of Biotechnology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi-26 124, Tamil Nadu, India.
2Post Graduate and Research Department of Botany, Pachaiyappa's College, Chennai-600 030, Tamil Nadu, India.
3Department of Zoology, Thiagarajar College (Autonomous), Madurai- 625 009, Tamil Nadu, India.


* Author for correspondence: T. Rajagopal
Post Graduate Department of Biotechnology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi-626 124, Tamil Nadu, India.
Also: Department of Zoology, Thiagarajar College (Autonomous), Madurai- 625 009, Tamil Nadu, India.
Email : This email address is being protected from spambots. You need JavaScript enabled to view it. Tel.: +91 452 2311875 Fax: +91 452 2312375

 

Abstract.

Justicia tranquebariensis L.f. is well known medicinal plants used in the treatment of respiratory problems. In the present study, a simple and reproducible protocol for rapid in vitro multiplication from nodal explants has been developed and randomly amplified polymorphic DNA (RAPD) markers were used for the first time for the detection of genetic polymorphism in this medicinal herb from in vitro grown plants with mother plants. Nodal explants of J. tranquebariensis showed better multiple shoot initiation. Among the different concentrations of 6-benzylaminopurine (BA), kinetin (KN), indole-3-acetic acid (IAA) and naphthalene acetic acid (NAA) tested, the maximum percentage of response (80%) was recorded on MS medium supplemented with KN (2.5 mg/l). Rooting was best achieved (80%) on half strength MS medium amended with IAA (1.0 mg/l). The plantlets regenerated in vitro with well developed shoots and roots were successfully transferred and established in plastic cups and pots containing soil mixture simultaneously with a 70% survival rate. Calli were induced from nodal and leaf explants with different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D), BA, NAA and optimum callus biomass (60%) was observed from leaf explants cultured on MS medium supplemented with 2, 4-D (1.0 mg/l). Ten arbitrary decamer primers have been used to amplify genomic DNA from in vitro raised field material and mother plant. Further, the genetic fidelity of micropropagated plants and callus was assessed by RAPD analysis and it showed that the similarity among the in vitro regenerated shoots and variation with the callus regenerated shoots. The results suggested that the multiple shoot induction and regeneration were regulated by appropriate cytokinin/auxin ratios rather than their relative concentrations. High multiplication frequency and molecular stability ensures the efficacy of the protocol developed for production and conservation of this important medicinal herb.

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