As. Pac. J. Mol. Biol. & Biotech., Jun 2017 Vol. 1, 12-17
Evaluation of total essential oil and cytotoxic activity of Barleria lupulina Lindl
Names Kanjana Sirisidthi1, Supriya Chabra2, Kanitta Jiraungkoorskul1, Wannee Jiraungkoorskul1*
1Department of Pathobiology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand 2Mahidol University International College, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand.
* Author for correspondence: Wannee Jiraungkoorskul
Department of Pathobiology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand.
Barleria lupulina Lindl. (Family: Acanthaceae) is a well-known perennial, distributed in the tropical Asia, Southeast Asia, and Africa. In this study, the leaves were aqueous extracted in various time. The extractions were determined the highest amount of total phenolic compound and used for evaluating the cytotoxicity test. The median (LC50) and 90% lethal concentration (LC90) against Artemia salina at varied concentrations of this plant extractions as 0, 400, 800, 1,600, 3,200, 6,400 and 12,800 ppm were evaluated within 24 hours exposure. The result revealed that the total phenolic compound measurements were 28.90+4.27, 40.87+3.85, 24.65+4.55, 13.13+2.36 and 20.26+2.25 mg gallic acid equivalent per g of extract in 0.5, 1, 3, 5 and 24 hours extractions, respectively. Due to the highest amount of total phenolic compound, the 1-hour aqueous extract of B. lupulina leaf expressed the 24-h LC50 and LC90 values against A. salina were 6,058.23 and 10,895.03 ppm, respectively.