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* Entrance Fee of RM20 is chargeable to first time applicants for all membership categories except for student membership.

* Membership is open only to those who qualify under the MPS Constitution and is subjected to approval by the Council.

 

Abstract Rhizospheric Cymbopogon citratus-Staphylococcus succinus Interactions for Phytoremediation of Dibenzofuran Contaminated Soil

Rhizospheric Cymbopogon citratus-Staphylococcus succinus Interactions for Phytoremediation of Dibenzofuran Contaminated Soil

Nurul Elyni Mat Shaari1, Md. Tajol Faeiz Md. Tajudin1, Zarina Zainudin1, Noor Faizul Hadry Nordin*

1Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia
*Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia
*International Institute For Halal Research And Training (Inhart), International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia


* Author for correspondence: Noor Faizul Hadry Nordin
International Institute For Halal Research And Training (Inhart), International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia
Emel : This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Chloroaromatic compounds such as dioxins and dioxin-like compounds is categorized as a highly persistent chemical and accumulates in the environment as well as increasing their distribution in our ecosystem. Phytoremediation is one of the tolerable ways that has been operated to battle out the problem of contaminants. This study investigated the lemongrass (Cymbopogon citratus) traits and soil enzyme activities upon dibenzofuran (DBF) pollution and inoculation of Staphylococcus succinus in soil. Lemongrass was planted into uncontaminated soil and DBF-contaminated soil. Four treatments were conducted; soil, soil with plant, soil with bacteria, and soil with plant and bacteria. After 30 days exposure, the plant traits such as plant length, biomass, chlorophyll content and soil enzymes activities were examined to investigate the sensitivity of lemongrass towards DBF. The presence of DBF in soil, plant length and aboveground and belowground mass were decreased compared to control. However, with inoculation of Staphylococcus succinus in soil, these plant traits were significantly higher than control, suppressing the effects of DBF. The chlorophyll content of lemongrass indicated slightly difference showing a less or no effect of DBF. Nevertheless, enzyme activities of asparaginase and protease exhibited higher activity than uncontaminated soil. DBF reduction can be seen by GC-MS analysis where symbiosis of lemongrass and bacteria showed the highest reduction percentage.

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Abstract Optimization of plant growth regulators for Citrus suhuiensis callus induction

Optimization of plant growth regulators for Citrus suhuiensis callus induction

Nur Alia M. Fathil1, Noor Illi Mohamad Puad1, Azura Amid1, Azlin Suhaida Azmi1, Rusli Ibrahim2

1Bioprocess and Molecular Engineering Research Unit (BPMERU), Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10, 50728 Kuala Lumpur, Malaysia.
2Agrotechnology and Biosciences Division, Malaysian Nuclear Agency, 43000 Bangi, Selangor, Malaysia.


* Author for correspondence: Noor Illi Mohamad Puad
Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10, 50728 Kuala Lumpur, Malaysia.
Fax: +603 6196 4442; Tel: +603 6196 5702 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Correct types and concentration of plant growth regulators (PGRs) will enhance and optimize the growth of callus cultures. This paper reported the effects of several types of cytokinins (2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthalene acetic acid (NAA)) and auxins (6–Benzylaminopurine (BAP) and kinetin) on the callus induction of C. suhuiensis. The cotyledons from C. suhuiensis seeds were excised as the explant and cultured on Murashige and Skoog (MS) media, 3% (w/v) sugar, 0.05% (w/v) malt extract and 0.25% (w/v) agar under the continuous dark condition supplemented with the chosen PGRs at the concentration range of 0.5, 1.0, 2.0, 3.0 and 4.0 mg/L. The growth of callus at each treatment was measured as gram (g) of fresh weight and percentage of callus induction. The results showed that 1.0 mg/L 2,4-D gave the highest growth of callus (0.15 g and 100% callus percentage). After identifying the effective PGRs, Central Composite Design (CCD) from the Design Expert® software version 9.0 was used to obtain the optimum concentration of cytokinin and auxin on C. suhuiensis callus cultures. It was observed that the highest amount of callus culture induced was 0.218 g when the media was supplemented with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin.

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Abstract Cytokinetic Study of Uninfected Agarwood Branch Ethanolic Extract on Breast Cancer Cells

Cytokinetic Study of Uninfected Agarwood Branch Ethanolic Extract on Breast Cancer Cells

Phirdaous Abbas1, Yumi Zuhanis Has-Yun Hashim1, Azura Amid1

1Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, Jalan Gombak, 53100 Kuala Lumpur.
2International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, P.O. Box 50728, Kuala Lumpur Malaysia


* Author for correspondence: Yumi Zuhanis Has-Yun Hashim
Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, Jalan Gombak, 53100 Kuala Lumpur.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Breast cancer is the most common malignancy in women with over 500,000 global death toll annually. Ethnopharmaceutical practices provide evidences of Aquilaria spp. as traditional treatment for numerous illness. This study intended to look into the cytotoxic kinetics of uninfected agarwood branch ethanolic extract from Aquilaria subintegra against MCF-7 breast cancer cell line that could help grasp the effect and provide baseline towards development of agarwood-based therapeutics. Extract was subjected to a series of kinetic studies in order to investigate the growth rate, death rate, doubling time and death time of MCF-7 between the treated and control groups. Results obtained indicated that the extract lowered the growth rate from 0.0421 h-1 to 0.0158 h-1 and stretched the doubling time from 16.4 hours to 43.8 hours. Death rate was escalated from 0.0058 h-1 to 0.0563 h-1. Closer inspection on the population density images revealed that the extract-treated MCF-7 cells showed abnormal appearance with blebs, volume reduction, cell shrinking and loss of cell-cell contact. These discoveries implied that agarwood uninfected branch ethanolic extract altered the kinetics of MCF-7 cells and additional study should be made to substantiate the hypothesis and elucidate the effects against cancer.

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Abstract Chlamydomonas reinhardtii as Host in Production of Recombinant Proteins por Medical Uses

Chlamydomonas reinhardtii as Host in Production of Recombinant Proteins por Medical Uses

Wan Hannah Hanim Wan Mohamad1, *Raha Ahmad Raus1, Zarina Zainuddin2, Noriha Mat Amin3, Norlia Basherudin4

1Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, 50728 Kuala Lumpur, Malaysia.
2Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
3Pusat Penyelidikan Bioteknologi, Ibu Pejabat MARDI, Serdang, Selangor, Malaysia
4Biotechnology Division, Forest Research Institute of Malaysia, Kepong, Selangor, Malaysia


* Author for correspondence: Raha Ahmad Raus
Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, 50728 Kuala Lumpur, Malaysia.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.*

 

Abstract.

Microalgae as a new preferred bioreactor system have many advantages compared with other bioreactor system as it is edible and does not contain endotoxins for human consumption. The green microalga species Chlamydomonas reinhardtii possesses many beneficial properties that have made it as model organism in photosynthetic related studies, biofuel production and also as eukaryotic host in heterologous proteins production. Recombinant proteins expressed in C. reinhardtii have been produced for the medical applications as hormones, vaccines, and antibodies. Production of these therapeutic proteins in microalgae has enhanced the yield of the proteins expressed compared to the traditional cell tissue culture methods which normally are more expensive in the cost of production. In this review, the recombinant microalgae developed by genetic transformation of both nuclear and chloroplast genomes are discussed, recombinant proteins that had been produced for medical purposes in C. reinhardtii are presented and the transformation methods as well as strategies for better protein expression in C. reinhardtii are also discussed.

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Abstract Effect of Carbon Sources and Plant Growth Regulators on Efficient Callus Induction and Regeneration in Barley (Hordeum vulgare L.)

As. Pac. J. Mol. Biol. & Biotech., Jun 2017 Vol. 1, 2-11

Effect of Carbon Sources and Plant Growth Regulators on Efficient Callus Induction and Regeneration in Barley (Hordeum vulgare L.)

S. M. Shahinul Islam* and Mozidul Haque

Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

* Author for correspondence: Dr. S. M. Shahinul Islam
Associate Professor, Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

An efficient callus induction and regeneration system has been developed for BARI barley-6 during this study. Seed-derived embryos were used as explant sources for callus induction. The highest frequency of callus induction (71.38%) was obtained when 3.5 mg/l 2,4-D was provided to seeds for 4 days prior to culture. The lowest callusing (15.55%) was recorded when the seeds were not pretreated with 2,4-D at all (negative control). Various carbohydrates (sucrose, maltose and D-sorbitol) either singly or in combination were added to three basal media, namely MS, B5 and N6. All media were also supplemented with 1.5 mg/l 2,4-D, 300 mg/l L-proline and 300 mg/l casein hydrolysate for improving callus induction. Different PGRs were used with MS medium and its effect on embryogenic callus induction and their subsequent regeneration was observed. 60 g/l D-sorbitol (T6) gave the highest frequency of primary callus induction (89.16%) in MS medium and 15 g/l maltose with 15 g/l D-sorbitol (T9) gave the lowest frequency (11.33%) in B5 medium. The difference between treatments with different carbohydrate sources and the tested media, with MS, N6 and B5 for callus induction, was significant at the level of P

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About MSMBB

We are a non-profit organisation that was established in 1988 to promote molecular biology and biotechnology.

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4th International Conference on Molecular Biology & Biotechnology

in conjunction with the 27th  Scientific Meeting of the Malaysian Society of Molecular Biology & Biotechnology (MSMBB) 

Beyond 2021: Building Sustainable Science

1-3rd June2021

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