As. Pac. J. Mol. Biol. & Biotech., Jan 2014 Vol. 1, 137-144
Effect of silver nitrate and gibberellic acid on in vitro regeneration, flower induction and fruit development in Naga Chilli
G. Bora1,2*, H. K. Gogoi1, P. J. Handique2
1Division of Biotechnology, Defence Research Laboratory, Tezpur-784001, Assam, India.
2Department of Biotechnology, Gauhati University, Guwahati-781014, India.
* Author for correspondence: Ms. Geetashree Bora
Christian Colony, Jail Road, Borbheta, Jorhat-785004, Assam, India.
In vitro flower induction and fruit development are rarely achieved in the genus Capsicum. This communication reports for the first time the induction of in vitro flower induction and fruit development in Bhot jolokia or Naga Chilli under the influence of silver nitrate (AgNO3) and gibberellic acid (GA3). The greatest number of multiple shoots from a single shoot apical meristem was induced in MS medium fortified with BAP (45µML-1), NAA (5.5µML-1) and AgNO3 (35µML-1). Half strength MS medium supplemented with AgNO3 at a concentration of 34µML-1 and GA3 at 28µML-1 was found to be the optimum concentration for in vitro flower induction, and AgNO3 at 32µML-1 added to half strength MS was found best for in vitro fruit development. In vitro regenerated shoots were provided with rooting medium containing MS medium, IBA and BAP at 3.5μML-1 and 2.5μML-1, respectively. Healthy rooted plantlets were transferred to potting substrate where half strength MS medium was enhanced with soil and vermicompost in equal volumes (1:1) for hardening in the poly house. 72.6% of all acclimatised plants were successfully transferred to the open field.