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Abstract Production Of Fused Protein Consisting Of Vp2 From Infectious Bursal Disease Virus And Hn From Newcastle Disease Virus In Escherichia Coli

Production Of Fused Protein Consisting Of Vp2 From Infectious Bursal Disease Virus And Hn From Newcastle Disease Virus In Escherichia Coli

Muhammad N.A.A.N.1, *Ahmad-Raus R.2, Amin N.M.3, Syamsiah A. S.4, Bakar F.D.A.5, Ghazali M.S.1

1Department of Biomedical Science, Faculty of Health Sciences, International Islamic University Malaysia
2Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia
3Malaysian Agricultural Research and Development Institute, Serdang, Malaysia
4Veterinary Research Institute, Ipoh, Malaysia
5School of Bioscience and Biotechnology, National University of Malaysia


* Author for correspondence: Raha Ahmad-Raus
Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University of Malaysia, Jalan Gombak, Selangor
Tel.:+603-61964588 E-mail address: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract.

Viral protein 2 (VP2) of infectious bursal disease virus (IBDV) and hemagglutinin-neuraminidase (HN) of Newcastle disease virus (NDV) are viral surface proteins which contain epitopes that are able to induce neutralizing antibodies for protection against Newcastle disease and infectious bursal disease. In the present study, recombinant fused protein consisting of VP2 and partial HN protein was produced. The fused protein was made by fusing VP2 gene to full length (flHN) and partial HN (pHN) gene, separately and cloned it into pRSETB expression vector. The recombinant construct is then transformed into E. coli BL21 (DE3) for production of the fused protein. The fused VP2-pHN protein was successfully produced in E. coli BL21 (DE3) strain with the size of 75 kDa detected by anti-His monoclonal antibody via Western blot analysis.

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