Current Issue

 

Volume 28(1); 2020

 

Nuclease pre-treatment increases efficiency of whole genome sequencing of Influenza B virus in respiratory specimens
Wudtichai Manasatienkij, Piyawan Chinnawirotpisan, Weerayuth Kittichotirat, Sriluck Simasathien, Louis R. Macareo, Damon W. Ellison, Supapon Cheevadhanarak, Wiriya Rutvisuttinunt, Stefan Fernandez, Chonticha Klungthong
APJMBB 28 (1): 1-13
Article DOI: https://doi.org/10.35118/apjmbb.2020.028.1.01
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The use of next generation sequencing (NGS) directly on respiratory specimens to obtain viral whole genome sequence (WGS) enhances the capability for rapid and unbiased viral characterization. One of the challenges of using NGS directly in influenza-like illness (ILI) respiratory specimens is the higher proportion of host and bacterial genome compared to viral genetic materials found, which reduces the likelihood of obtaining complete viral genome sequences. This study aims to evaluate nuclease pretreatments prior to sequencing of influenza B virus directly from ILI respiratory specimens.  Sequence data were mapped to human, bacteria and influenza B viral genome. In the absence of any nuclease pretreatments, the sequence reads identified as Haemophilus influenzae, Haemophilus parainfluenzae, Neisseria meningitidis and Veillonella parvula were the most prominent genetic materials in respiratory specimens. Filtration followed by nuclease treatment reduced bacterial sequence reads by at least 70 folds in all 4 tested samples, supporting the direct application of NGS in ILI respiratory specimens. Although the pretreatment methods significantly reduced human genome sequences, the remaining human genome especially human rRNA still impact the number and proportion of the viral sequence reads.
Potential use of Pennisetum purpureum for phytoremediation and bioenergy production: a mini review
Nurul Atiqah Osman, Ahmad Muhaimin Roslan, Mohamad Faizal Ibrahim, Mohd Ali Hassan
APJMBB 28 (1): 14-26
Article DOI: https://doi.org/10.35118/apjmbb.2020.028.1.02
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Organic and/or heavy metal pollutants in soil and wastewater can be remediated by phytoremediation. Phytoremediation combines the disciplines of plant physiology, soil microbiology and soil chemistry. There are several ways by which plants extract, stabilize, filtrate, volatilize or degrade the contaminants. However, the effectiveness of phytoremediation relies upon the type of plant used. Pennisetum purpureum, commonly referred to as Napier grass, is one of the exceptional phytoremediators due to its rapid growth rate and ability to survive in highly contaminated soils. In the present review, the potential use and applicability of P. purpureum to remediate various contaminated areas was highlighted and comprehensively discussed, especially the five phytoremediation mechanisms involved (i.e., phytodegradation, phytoextraction, phytofiltration, phytostabilization, phytovolatilization). The application and management of P. purpureum
in soil and wastewater phytoremediation were also critically presented. The coupling of phytoremediation and bioenergy is the zero-waste concept that can be applied since P. purpureum contains high lignocellulosic content that can be utilized as carbon source for biofuel production, such as ethanol and butanol.

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3rd International Conference on Molecular Biology & Biotechnology

in conjunction with the 26th  Scientific Meeting of the Malaysian Society of Molecular Biology & Biotechnology (MSMBB) & UCSI University's 2nd Applied Science Symposium

Organized by MSMBB & UCSI University

FOSTERING INNOVATION THROUGH RESEARCH

24-25th April 2019, UCSI University Kuala Lumpur

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